In this work, we use microfluidic impedance cytometry to investigate the interactions of antimicrobial peptides (AMPs) with bacteria and human cells. our findings reveal characteristic alterations in the bacteria/cell electrical fingerprints following exposure to AMPs at different concentrations. specifically, the electric phase at 11 MHz turns out to be a promising label-free metric for monitoring AMPs bactericidal activity and toxicity to host cells.
Troiano, C., De Ninno, A., Casciaro, B., Riccitelli, F., Bobone, S., Massoud, R., et al. (2022). SINGLE-CELL IMPEDANCE CYTOMETRY TO EXPLORE THE ACTION OF ANTIMICROBIAL PEPTIDES. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp.486-487). Chemical and Biological Microsystems Society.
SINGLE-CELL IMPEDANCE CYTOMETRY TO EXPLORE THE ACTION OF ANTIMICROBIAL PEPTIDES
Troiano C.;De Ninno A.;Riccitelli F.;Bobone S.;Massoud R.;Bisegna P.;Stella L.;Caselli F.
2022-01-01
Abstract
In this work, we use microfluidic impedance cytometry to investigate the interactions of antimicrobial peptides (AMPs) with bacteria and human cells. our findings reveal characteristic alterations in the bacteria/cell electrical fingerprints following exposure to AMPs at different concentrations. specifically, the electric phase at 11 MHz turns out to be a promising label-free metric for monitoring AMPs bactericidal activity and toxicity to host cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
