Reactive oxygen species (ROS) are involved in many forms of apoptosis and mediate apoptosis in a number of cell types. In this paper, we use a variant of U937 monocytic cells (U937 HX) that show different biochemical features with respect to standard U937. Apoptotic standard U937 extrude reduced glutathione (GSH) and generate free radicals concomitantly with loss of mitochondria transmembrane potential (mt DeltaPsi). These events are correlated with the extrusion of intracellular GSH. Conversely, apoptotic U937 HX cells retain GSH, and the loss of mt DeltaPsi is not accompanied by generation of free radicals. The perfect inverse correlation between (a) ROS generation and (b) the presence of intracellular GSH during apoptosis suggests novel mechanisms to finely tune ROS generation in apoptosis.

D'Alessio, M., Cerella, C., De Nicola, M., Bergamaschi, A., Magrini, A., Gualandi, G., et al. (2003). Apoptotic GSH extrusion is associated with free radical generation. In Annals of the New York Academy of Sciences (pp.449-452) [10.1196/annals.1299.082].

Apoptotic GSH extrusion is associated with free radical generation

MAGRINI, ANDREA;GHIBELLI, LINA
2003-01-01

Abstract

Reactive oxygen species (ROS) are involved in many forms of apoptosis and mediate apoptosis in a number of cell types. In this paper, we use a variant of U937 monocytic cells (U937 HX) that show different biochemical features with respect to standard U937. Apoptotic standard U937 extrude reduced glutathione (GSH) and generate free radicals concomitantly with loss of mitochondria transmembrane potential (mt DeltaPsi). These events are correlated with the extrusion of intracellular GSH. Conversely, apoptotic U937 HX cells retain GSH, and the loss of mt DeltaPsi is not accompanied by generation of free radicals. The perfect inverse correlation between (a) ROS generation and (b) the presence of intracellular GSH during apoptosis suggests novel mechanisms to finely tune ROS generation in apoptosis.
5th International Conference on Apoptosis from Signalling Pathways to Therapeutic Tools
LUXEMBOURG, LUXEMBOURG
JAN 29-FEB 01, 2003
Rilevanza internazionale
2003
Settore MED/44 - MEDICINA DEL LAVORO
English
Apoptosis; Glutathione; Mitochondrial transmembrane potential; Reactive oxygen species; ROS
free radical; glutathione; reactive oxygen metabolite; apoptosis; biochemical composition; cell level; conference paper; controlled study; correlation analysis; human; human cell; membrane potential; mitochondrial membrane; Apoptosis; Cell Hypoxia; Free Radicals; Glutathione; Humans; Intracellular Membranes; Membrane Potentials; Mitochondria; Puromycin; Reactive Oxygen Species; U937 Cells
Intervento a convegno
D'Alessio, M., Cerella, C., De Nicola, M., Bergamaschi, A., Magrini, A., Gualandi, G., et al. (2003). Apoptotic GSH extrusion is associated with free radical generation. In Annals of the New York Academy of Sciences (pp.449-452) [10.1196/annals.1299.082].
D'Alessio, M; Cerella, C; De Nicola, M; Bergamaschi, A; Magrini, A; Gualandi, G; Alfonsi, A; Ghibelli, L
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66738
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 25
social impact