Objectives: To determine the kinetics and the relationship between the T-cell receptor V beta (TCRBV) complementary determining region 3 length, the CD4 T-cell count and HIV viral load changes in HIV-1 infected infants treated early with highly active antiretroviral therapy (HAART) during 1 year of follow-up. Design: Two HIV-1 vertically infected infants, two HIV-1 vertically exposed uninfected and two healthy controls were analysed by spectratyping. Evaluation of viral load, CD4 naive and memory cell counts and a proliferation test were also carried out. Methods: Twenty-six families and subfamilies of the TCR on CD4 and CD8 T cells were analyzed by spectratyping. Flow cytometric analysis on peripheral blood mononuclear cells for CD4CD45Ra, CD4CD45Ro, CD8CD38, proliferation tests and plasma viral load measurements were performed at baseline, 1, 6 and after 12 months of therapy. Results: HAART induced a marked reduction of viral load in both HIV-1 infected infants and an increase to normal CD4 T-cell count in the symptomatic infant. At baseline the TCRBV family distribution in the majority of CD8 and a few of the CD4 T cells was highly perturbed, with several TCRBV families showing a monoclonal/ oligoclonal distribution. During HAART a normalization of the TCR repertoire in both CD8 and CD4 subsets occurred. TCR repertoire normalization was associated with a good virological and immunological response. Conclusion: These results suggest that complete and early virus replication control as a result of early HAART leads to a marked reduction of T-cell oligoclonality and is an essential prerequisite to the development of a polyclonal immune response in HIV-1 infected infants. (C) 2001 Lippincott Williams & Wilkins.

Romiti, M.l., Cancrini, C., Castelli Gattinara, G., Di Cesare, S., Ciaffi, P., Bernardi, S., et al. (2001). Kinetics of the T-cell receptor CD4 and CD8 V beta repertoire in HIV-1 vertically infected infants early treated with HAART. AIDS, 15(16), 2075-2084 [10.1097/00002030-200111090-00002].

Kinetics of the T-cell receptor CD4 and CD8 V beta repertoire in HIV-1 vertically infected infants early treated with HAART

CANCRINI, CATERINA;ROSSI, PAOLO
2001-01-01

Abstract

Objectives: To determine the kinetics and the relationship between the T-cell receptor V beta (TCRBV) complementary determining region 3 length, the CD4 T-cell count and HIV viral load changes in HIV-1 infected infants treated early with highly active antiretroviral therapy (HAART) during 1 year of follow-up. Design: Two HIV-1 vertically infected infants, two HIV-1 vertically exposed uninfected and two healthy controls were analysed by spectratyping. Evaluation of viral load, CD4 naive and memory cell counts and a proliferation test were also carried out. Methods: Twenty-six families and subfamilies of the TCR on CD4 and CD8 T cells were analyzed by spectratyping. Flow cytometric analysis on peripheral blood mononuclear cells for CD4CD45Ra, CD4CD45Ro, CD8CD38, proliferation tests and plasma viral load measurements were performed at baseline, 1, 6 and after 12 months of therapy. Results: HAART induced a marked reduction of viral load in both HIV-1 infected infants and an increase to normal CD4 T-cell count in the symptomatic infant. At baseline the TCRBV family distribution in the majority of CD8 and a few of the CD4 T cells was highly perturbed, with several TCRBV families showing a monoclonal/ oligoclonal distribution. During HAART a normalization of the TCR repertoire in both CD8 and CD4 subsets occurred. TCR repertoire normalization was associated with a good virological and immunological response. Conclusion: These results suggest that complete and early virus replication control as a result of early HAART leads to a marked reduction of T-cell oligoclonality and is an essential prerequisite to the development of a polyclonal immune response in HIV-1 infected infants. (C) 2001 Lippincott Williams & Wilkins.
2001
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA
English
HAART; Immune reconstitution; Paediatrics; T-cell repertoire; Vertical HIV-1 infection
antiretrovirus agent; CD4 antigen; CD8 antigen; zidovudine; article; binding kinetics; case report; cell proliferation; cellular distribution; complementarity determining region; controlled study; female; flow cytometry; follow up; human; human cell; Human immunodeficiency virus 1; Human immunodeficiency virus infection; immune response; infant; infant disease; lymphocyte count; male; memory cell; mononuclear cell; peripheral lymphocyte; polyclonal activation; priority journal; receptor binding; spectroscopy; T lymphocyte activation; T lymphocyte receptor gene; vertical transmission; virus load; virus replication; Antigens, CD3; Antiretroviral Therapy, Highly Active; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Disease Transmission, Vertical; Flow Cytometry; HIV Infections; HIV-1; Humans; Infant; Kinetics; Lymphocyte Activation; Receptors, Antigen, T-Cell, alpha-beta; Treatment Outcome; Viral Load
Romiti, M.l., Cancrini, C., Castelli Gattinara, G., Di Cesare, S., Ciaffi, P., Bernardi, S., et al. (2001). Kinetics of the T-cell receptor CD4 and CD8 V beta repertoire in HIV-1 vertically infected infants early treated with HAART. AIDS, 15(16), 2075-2084 [10.1097/00002030-200111090-00002].
Romiti, Ml; Cancrini, C; Castelli Gattinara, G; Di Cesare, S; Ciaffi, P; Bernardi, S; De Gasperi, Mr; Halapi, E; Rossi, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/57604
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