In the clinical practice, the hemodialysis adequacy was assessed by Kt/Vurea, a mathematical model to quantify the urea clearance on a single hemodialysis session. Glutathione-S-Transferases (GST) represent a superfamily of ubiquitous enzymes devoted to the cell protection and they are thought to play a role in the detoxification of both endogenous and exogenous compounds. Previous study demonstrated an increased e-GST activity in uremic patients.Because of hemodialysis is a “detoxification” therapy, we hypothesize that there may be observe different e-GST activity levels with different techniques and/or dialytic doses in uremic population undergoing to dialysis. The aims of the study are to compare e-GST activity in normal and uremic subjects, and to correlate the dialytic dose and the hemodialysis technique (convective and diffusive) with e-GST activity. Materials and methods: e-GST activity was assayed using a new automated procedure. 103 uremic patients divided into two groups basis on dialytic procedures 44 out of 103 patients underwent to standard bicarbonate HemoDialysis (HD-group); 59 patients were treated with online-HemoDiaFiltration therapy (HDF-group) 62 MHD patients and 80 healthy subjects (control group) were studied. Results: Comparing the e-GST activities of the control group (5.6±1.7 U/grHb) versus all uremic patients (9.0±3.1 U/grHb) , we observed a significant statistically difference (p<0.0001). Moreover, we observed statistical significant differences for e-GST activity (p=0.0036), Kt/Vurea (p=0.0007) and weekly Kt/Vurea (p=0.0004) in two subgroups of uremic patients. To try to distinguish between dialytic technique and Kt/V as the cause of the different e-GST expression, we divided all 103 hemodialytic patients in two subgroups using 1.3 as cut-off value of Kt/Vurea. In the patients with Kt/Vurea <1,3 (n° pts) e-GST was 9,67±3,23 while in patients with Kt/Vurea ≥1.3 was 8,65± 2,96, without any statistically significant difference (p=0,156). Conclusions: This preliminary study will be confirmed by a large trial. In fact a very large number of patients need to highlight the eGST as a long term marker of detoxification, such as an “glycate haemoglobin” for the dialysis therapy.

Noce, A., Ferrannini, M., Dessi', M., Fabrini, R., Palumbo, R., Ricci, G., et al. (2011). impact of hemodialytic procedures and dialytic doses on erythrocyte glutathione s-tranferase (e-gst) activity. In The International Journal of Artificials Organs (pp.672-673). Wichtig Editor.

impact of hemodialytic procedures and dialytic doses on erythrocyte glutathione s-tranferase (e-gst) activity

Noce, A;DESSI', MARIARITA;RICCI, GIORGIO;DI DANIELE, NICOLA
2011-10-01

Abstract

In the clinical practice, the hemodialysis adequacy was assessed by Kt/Vurea, a mathematical model to quantify the urea clearance on a single hemodialysis session. Glutathione-S-Transferases (GST) represent a superfamily of ubiquitous enzymes devoted to the cell protection and they are thought to play a role in the detoxification of both endogenous and exogenous compounds. Previous study demonstrated an increased e-GST activity in uremic patients.Because of hemodialysis is a “detoxification” therapy, we hypothesize that there may be observe different e-GST activity levels with different techniques and/or dialytic doses in uremic population undergoing to dialysis. The aims of the study are to compare e-GST activity in normal and uremic subjects, and to correlate the dialytic dose and the hemodialysis technique (convective and diffusive) with e-GST activity. Materials and methods: e-GST activity was assayed using a new automated procedure. 103 uremic patients divided into two groups basis on dialytic procedures 44 out of 103 patients underwent to standard bicarbonate HemoDialysis (HD-group); 59 patients were treated with online-HemoDiaFiltration therapy (HDF-group) 62 MHD patients and 80 healthy subjects (control group) were studied. Results: Comparing the e-GST activities of the control group (5.6±1.7 U/grHb) versus all uremic patients (9.0±3.1 U/grHb) , we observed a significant statistically difference (p<0.0001). Moreover, we observed statistical significant differences for e-GST activity (p=0.0036), Kt/Vurea (p=0.0007) and weekly Kt/Vurea (p=0.0004) in two subgroups of uremic patients. To try to distinguish between dialytic technique and Kt/V as the cause of the different e-GST expression, we divided all 103 hemodialytic patients in two subgroups using 1.3 as cut-off value of Kt/Vurea. In the patients with Kt/Vurea <1,3 (n° pts) e-GST was 9,67±3,23 while in patients with Kt/Vurea ≥1.3 was 8,65± 2,96, without any statistically significant difference (p=0,156). Conclusions: This preliminary study will be confirmed by a large trial. In fact a very large number of patients need to highlight the eGST as a long term marker of detoxification, such as an “glycate haemoglobin” for the dialysis therapy.
Congress of the European Society for Artificial Organs
Porto (Portogallo)
2011
38.
Rilevanza internazionale
su invito
10-ott-2011
ott-2011
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Glutathione-S-Transferases, uremic patients, hemodialysis
Intervento a convegno
Noce, A., Ferrannini, M., Dessi', M., Fabrini, R., Palumbo, R., Ricci, G., et al. (2011). impact of hemodialytic procedures and dialytic doses on erythrocyte glutathione s-tranferase (e-gst) activity. In The International Journal of Artificials Organs (pp.672-673). Wichtig Editor.
Noce, A; Ferrannini, M; Dessi', M; Fabrini, R; Palumbo, R; Ricci, G; DI DANIELE, N
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/55454
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