We demonstrated that neuroblastoma cells transfected with the pro-oxidant mutant G93A Cu,Zn SOD, accumulated oxidized proteins as products of an increased production of reactive oxygen species (ROS). However, cells survive by raising the activity of the proteasome proteolytic system. Proteasome activation also resulted in increased nNOS turnover thus preventing NO production. Chemical proteasome inhibition by lactacystin led to higher production of NO and accumulation of oxidized proteins that triggered cell death by apoptosis. DMPO, a well-known antioxidant agent, inhibited increased oxidized proteins formation and, at the same time, lowered proteasome activity to basal levels.
Aquilano, K., Rotilio, G., Ciriolo, M.r. (2003). Role of proteasome in counteracting reactive oxygen and nitrogen species-mediated damage in neuroblastoma cells. In PROCEEDINGS OF THE MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH, EUROPEAN SECTION - FREE RADICALS AND OXIDATIVE STRESS: CHEMISTRY, BIOCHEMISTRY AND PATHOPHYSIOLOGICAL IMPLICATIONS.
Role of proteasome in counteracting reactive oxygen and nitrogen species-mediated damage in neuroblastoma cells
AQUILANO, KATIA;ROTILIO, GIUSEPPE;CIRIOLO, MARIA ROSA
2003-01-01
Abstract
We demonstrated that neuroblastoma cells transfected with the pro-oxidant mutant G93A Cu,Zn SOD, accumulated oxidized proteins as products of an increased production of reactive oxygen species (ROS). However, cells survive by raising the activity of the proteasome proteolytic system. Proteasome activation also resulted in increased nNOS turnover thus preventing NO production. Chemical proteasome inhibition by lactacystin led to higher production of NO and accumulation of oxidized proteins that triggered cell death by apoptosis. DMPO, a well-known antioxidant agent, inhibited increased oxidized proteins formation and, at the same time, lowered proteasome activity to basal levels.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
