In vitro HIV-1 infection induced a significant decrease in intracellular reduced glutathione (GSH) in human macrophages, Such a decrease was observed at the time of infection corresponding to maximum release of virus from infected cells and was not related to cell cytotoxicity, GSH loss was not related to its oxidation or leakage through the cell membrane, Inhibition of intracellular GSH synthesis by buthionine sulfoximine (BSO) did not further decrease GSH levels with respect to the decrease caused by HIV alone. However, treatment of macrophages with BSO significantly increased the HIV yield in the supernatant. Exogenous GSH strongly suppressed the production of p24 gag protein as well as the virus infectivity. Previous observations with other RNA and DNA viruses consistently showed that GSH antiviral effect occurred at late stages of virus replication and was related to the selective decrease of specific glycoproteins, such as gp120, which are particularly rich in disulfide bonds.
|Autori interni:||GARACI, ENRICO |
CIRIOLO, MARIA ROSA
|Autori:||Garaci E., Palamara A.T., Ciriolo M.R., D'Agostini C., Ab Del-Latif M.S., Aquaro S., Lafavia E., Rotilio G.|
|Titolo:||Intracellular GSH content and HIV replication in human macrophages|
|Nome del convegno:||3rd International Workshop on HIV and Cells of Macrophage Lineage|
|Luogo del convegno:||VERONA, ITALY|
|Anno del convegno:||OCT 16-19, 1996|
|Data di pubblicazione:||1997|
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
|Tipologia:||Intervento a convegno|
|Citazione:||Garaci E., P.A. (1997). Intracellular GSH content and HIV replication in human macrophages. In Journal of Leukocyte Biology (pp.54-59). BETHESDA : FEDERATION AMER SOC EXP BIOL.|
|Appare nelle tipologie:||02 - Intervento a convegno|
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