Short-term incomplete cerebral ischemia was induced in the rat by bilaterally clamping for 5 min the common carotid arteries; subsequent reperfusion of 10 min was obtained by removing carotid occlusion. At the end of ischemia or reperfusion, animals were sacrificed by decapitation. A control group was represented by sham-operated rats. Peripheral venous blood samples were withdrawn from the femoral vein from rats subjected to cerebral reperfusion 5 min before ischemia, at the end of ischemia, and 10 min after reperfusion. A highly sensitive HPLC method for the direct determination of malondialdehyde, oxypurines, and nucleosides was used on 200 μL of brain tissue and plasma extracts. Incomplete cerebral ischemia induced the appearance of a significant amount of tissue malondialdehyde (undetectable in control animals) and a decrease of ascorbic acid. A further 6.6-fold increase of malondialdehyde and a 18.5% decrease of ascorbic acid occurred after 10 min of reperfusion. Plasma malondialdehyde, which was present in minimal amount before ischemia, significantly increased after 5 min of ischemia, being strikingly augmented after 10 min of reperfusion. A similar trend was observed for oxypurines and nucleosides. From these data, it can be affirmed that tissue concentrations of malondialdehyde and ascorbic acid, and plasma levels of malondialdehyde, oxypurines, and nucleosides, reflect both the oxygen radical-mediated tissue injury and the depression of energy metabolism, thus representing early biochemical markers of short-term incomplete brain ischemia and reperfusion in the rat.

Vagnozzi, R., Lazzarino, G., Tavazzi, B., Di Pierro, D., Siragusa, P., Giuffre, R., et al. (1995). Incomplete cerebral ischemia in the rat provokes increase of tissue and plasma malondialdehyde. In Biological Trace Element Research (pp.241-246). TOTOWA : HUMANA PRESS INC.

Incomplete cerebral ischemia in the rat provokes increase of tissue and plasma malondialdehyde

VAGNOZZI, ROBERTO;
1995-01-01

Abstract

Short-term incomplete cerebral ischemia was induced in the rat by bilaterally clamping for 5 min the common carotid arteries; subsequent reperfusion of 10 min was obtained by removing carotid occlusion. At the end of ischemia or reperfusion, animals were sacrificed by decapitation. A control group was represented by sham-operated rats. Peripheral venous blood samples were withdrawn from the femoral vein from rats subjected to cerebral reperfusion 5 min before ischemia, at the end of ischemia, and 10 min after reperfusion. A highly sensitive HPLC method for the direct determination of malondialdehyde, oxypurines, and nucleosides was used on 200 μL of brain tissue and plasma extracts. Incomplete cerebral ischemia induced the appearance of a significant amount of tissue malondialdehyde (undetectable in control animals) and a decrease of ascorbic acid. A further 6.6-fold increase of malondialdehyde and a 18.5% decrease of ascorbic acid occurred after 10 min of reperfusion. Plasma malondialdehyde, which was present in minimal amount before ischemia, significantly increased after 5 min of ischemia, being strikingly augmented after 10 min of reperfusion. A similar trend was observed for oxypurines and nucleosides. From these data, it can be affirmed that tissue concentrations of malondialdehyde and ascorbic acid, and plasma levels of malondialdehyde, oxypurines, and nucleosides, reflect both the oxygen radical-mediated tissue injury and the depression of energy metabolism, thus representing early biochemical markers of short-term incomplete brain ischemia and reperfusion in the rat.
4th International Congress on Trace Elements in Medicine and Biology - Trace Elements and Free Radicals in Oxidative Diseases
CHAMONIX, FRANCE
APR 05-09, 1993
MINIST RECH & TECHNOL, INSERM, SOC FRANCAISE BIOL CLIN, CLUB ETUDES RADICAUX LIBRES BIOL, JOSEPH FOURIER UNIV, GRENOBLE UNIV HOSP A MICHALLON, SOC FRANCOPHONE ETUDES & RECH ELEMENTS TRACE ESSENTIALS, SOC FREE RADICAL RES
Rilevanza internazionale
contributo
1995
Settore MED/27 - NEUROCHIRURGIA
English
ascorbic acid; malonaldehyde; nucleoside; purine derivative; animal model; animal tissue; artery clamp; brain ischemia; brain tissue; common carotid artery; conference paper; controlled study; high performance liquid chromatography; male; nonhuman; rat; reperfusion; Animal; Ascorbic Acid; Brain; Chromatography, High Pressure Liquid; Ischemic Attack, Transient; Lipid Peroxidation; Male; Malondialdehyde; Nucleosides; Purines; Rats; Rats, Wistar; Reference Values; Reperfusion; Sensitivity and Specificity; Time Factors
Intervento a convegno
Vagnozzi, R., Lazzarino, G., Tavazzi, B., Di Pierro, D., Siragusa, P., Giuffre, R., et al. (1995). Incomplete cerebral ischemia in the rat provokes increase of tissue and plasma malondialdehyde. In Biological Trace Element Research (pp.241-246). TOTOWA : HUMANA PRESS INC.
Vagnozzi, R; Lazzarino, G; Tavazzi, B; Di Pierro, D; Siragusa, P; Giuffre, R; Giardina, B
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49554
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 4
social impact