We have previously demonstrated that wild-type herpes simplex virus type 1 (HSV-1), as well as nonreplicating UV-inactivated HSV-1, promptly activates the nuclear factor-kappa B (NF-kappa B) in U937 monocytoid cells and that glycoprotein D (gD) of HSV-1 is sufficient by itself to exert a similar effect. We then investigated the signaling pathway used by HSV-1 to initiate NF-kappa B activation and, particularly, whether our observation could be related to the capability of HSV-1-gD to directly stimulate NF-kappa B through its interaction with the herpes virus entry receptor A (HveA). Here we report that: (a) co-cultivation of U937 cells with an adherent cell line expressing wild-type gD on its surface led to increased NF-kappa B activation, while co-cultivation with the same adherent cell line expressing a mutated form of gD, lacking the capability to bind HveA, did not cause the same effect; (b) exposure to UV-inactivated HSV-1 induced the activation of NF-kappa B in HveA-expressing U937 and THP-1 cells, but not in non-HveA-expressing HEp-2 cells; and (c) activation of NF-KB in U937 and THP-1 cells exposed to soluble gD was inhibited by an antibody able to interfere with gD-HveA interaction. These results suggest that HSV1-gD-HveA interaction initiates a signal transduction pathway leading to NF-kappa B activation.

Sciortino, M.t., Medici, M.a., Marino Merlo, F., Zaccaria, D., Giuffre, M., Venuti, A., et al. (2007). Signaling pathway used by HSV-1 to induce NF-kappa B activation - Possible role of herpes virus entry receptor A. In SIGNAL TRANSDUCTION PATHWAYS, PT D - INFLAMMATORY SIGNALING PATHWAYS AND NEUROPATHOLOGY (pp.89-96). OXFORD : BLACKWELL PUBLISHING [10.1196/annals.1397.074].

Signaling pathway used by HSV-1 to induce NF-kappa B activation - Possible role of herpes virus entry receptor A

GRELLI, SANDRO;MASTINO, ANTONIO
2007-01-01

Abstract

We have previously demonstrated that wild-type herpes simplex virus type 1 (HSV-1), as well as nonreplicating UV-inactivated HSV-1, promptly activates the nuclear factor-kappa B (NF-kappa B) in U937 monocytoid cells and that glycoprotein D (gD) of HSV-1 is sufficient by itself to exert a similar effect. We then investigated the signaling pathway used by HSV-1 to initiate NF-kappa B activation and, particularly, whether our observation could be related to the capability of HSV-1-gD to directly stimulate NF-kappa B through its interaction with the herpes virus entry receptor A (HveA). Here we report that: (a) co-cultivation of U937 cells with an adherent cell line expressing wild-type gD on its surface led to increased NF-kappa B activation, while co-cultivation with the same adherent cell line expressing a mutated form of gD, lacking the capability to bind HveA, did not cause the same effect; (b) exposure to UV-inactivated HSV-1 induced the activation of NF-kappa B in HveA-expressing U937 and THP-1 cells, but not in non-HveA-expressing HEp-2 cells; and (c) activation of NF-KB in U937 and THP-1 cells exposed to soluble gD was inhibited by an antibody able to interfere with gD-HveA interaction. These results suggest that HSV1-gD-HveA interaction initiates a signal transduction pathway leading to NF-kappa B activation.
Cell Signaling World 2006 Conference
Luxembourg, LUXEMBOURG
JAN 25-28, 2006
Rilevanza internazionale
su invito
gen-2007
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Herpes virus entry receptor; HSV-1; HveA; NF-κB
Intervento a convegno
Sciortino, M.t., Medici, M.a., Marino Merlo, F., Zaccaria, D., Giuffre, M., Venuti, A., et al. (2007). Signaling pathway used by HSV-1 to induce NF-kappa B activation - Possible role of herpes virus entry receptor A. In SIGNAL TRANSDUCTION PATHWAYS, PT D - INFLAMMATORY SIGNALING PATHWAYS AND NEUROPATHOLOGY (pp.89-96). OXFORD : BLACKWELL PUBLISHING [10.1196/annals.1397.074].
Sciortino, Mt; Medici, Ma; Marino Merlo, F; Zaccaria, D; Giuffre, M; Venuti, A; Grelli, S; Mastino, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/41355
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