A multichannel, “Barrier-on-a-Chip” platform with integrated impedance analysis has been developed. The device enables real-time monitoring of epithelial barrier integrity at the air-liquid interface (ALI) whilst minimising disruption to the physiological environment. Multi-frequency impedance data (1 kHz to 100 kHz) is collected every 17 minutes via custom made hardware. Data is fitted to an equivalent electrical circuit model to extract cell barrier parameters, such as cell barrier resistance (transepithelial resistance, TER) and capacitance. The system capability was demonstrated by monitoring the growth and formation of an epithelial barrier using human bronchial epithelial and human gut epithelial separately cells over 5 days, followed by barrier disruption using a viral mimetic.
Fernandes, J., Karra, N., Bowring, J., Reale, R., Swindle, E., Morgan, H. (2021). 'Barrier-on-a-chip' for real time impedance monitoring of epithelial barrier function. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) (pp.475-476). Chemical and Biological Microsystems Society.
'Barrier-on-a-chip' for real time impedance monitoring of epithelial barrier function
Reale R.;
2021-01-01
Abstract
A multichannel, “Barrier-on-a-Chip” platform with integrated impedance analysis has been developed. The device enables real-time monitoring of epithelial barrier integrity at the air-liquid interface (ALI) whilst minimising disruption to the physiological environment. Multi-frequency impedance data (1 kHz to 100 kHz) is collected every 17 minutes via custom made hardware. Data is fitted to an equivalent electrical circuit model to extract cell barrier parameters, such as cell barrier resistance (transepithelial resistance, TER) and capacitance. The system capability was demonstrated by monitoring the growth and formation of an epithelial barrier using human bronchial epithelial and human gut epithelial separately cells over 5 days, followed by barrier disruption using a viral mimetic.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
