Garlic extracts, either aqueous or oily, are commonly employed to prepare garlic derivative supplements used as nutraceuticals, for the treatment of different pathologies. In this study, we investigated the effects of water garlic extracts from two different areas of Italy well known for garlic production, Latina (GEL) and Sulmona (GES), on cell cycle and death of HepG2 hepatoma cells. The effects of the treatments with GEL and GES were also compared with the oil-soluble sulfur compound of garlic, diallyl disulfide (DADS). GEL and GES induced a p53/p21-dependent cell cycle arrest in G2/M phase and apoptosis, although to a different extent, whereas DADS, under the experimental conditions used, was not detrimental to HepG2 cells. GEL and GES committed HepG2 cells to apoptosis by the activation of cJun-NH2 terminal kinase (JNK)/c-Jun phosphorylative cascade without a detectable increase in the flux of reactive oxygen species. Moreover, differentiation of HepG2 cells induced by retinoic acid determined resistance to GEL and GES treatments without the activation of JNK signaling pathway. Overall, the results obtained indicate that water-soluble garlic extracts are more inhibitory of the growth of transformed hepatoma cells than the oil-soluble isolated compound DADS, and that their antiproliferative properties are different depending on the area of origin of the starting material. (c) 2006 Elsevier Inc. All rights reserved.

DE MARTINO, A., Filomeni, G., Aquilano, K., Ciriolo, M.r., Rotilio, G. (2006). Effects of water garlic extracts on cell cycle and viability of HepG2 hepatoma cells. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 17(11), 742-749 [10.1016/j.jnutbio.2005.12.005].

Effects of water garlic extracts on cell cycle and viability of HepG2 hepatoma cells

DE MARTINO, ANGELO;FILOMENI, GIUSEPPE;AQUILANO, KATIA;CIRIOLO, MARIA ROSA;ROTILIO, GIUSEPPE
2006-01-01

Abstract

Garlic extracts, either aqueous or oily, are commonly employed to prepare garlic derivative supplements used as nutraceuticals, for the treatment of different pathologies. In this study, we investigated the effects of water garlic extracts from two different areas of Italy well known for garlic production, Latina (GEL) and Sulmona (GES), on cell cycle and death of HepG2 hepatoma cells. The effects of the treatments with GEL and GES were also compared with the oil-soluble sulfur compound of garlic, diallyl disulfide (DADS). GEL and GES induced a p53/p21-dependent cell cycle arrest in G2/M phase and apoptosis, although to a different extent, whereas DADS, under the experimental conditions used, was not detrimental to HepG2 cells. GEL and GES committed HepG2 cells to apoptosis by the activation of cJun-NH2 terminal kinase (JNK)/c-Jun phosphorylative cascade without a detectable increase in the flux of reactive oxygen species. Moreover, differentiation of HepG2 cells induced by retinoic acid determined resistance to GEL and GES treatments without the activation of JNK signaling pathway. Overall, the results obtained indicate that water-soluble garlic extracts are more inhibitory of the growth of transformed hepatoma cells than the oil-soluble isolated compound DADS, and that their antiproliferative properties are different depending on the area of origin of the starting material. (c) 2006 Elsevier Inc. All rights reserved.
2006
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Apoptosis; Diallyl disulfide; Garlic; JNK; p53
DE MARTINO, A., Filomeni, G., Aquilano, K., Ciriolo, M.r., Rotilio, G. (2006). Effects of water garlic extracts on cell cycle and viability of HepG2 hepatoma cells. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 17(11), 742-749 [10.1016/j.jnutbio.2005.12.005].
DE MARTINO, A; Filomeni, G; Aquilano, K; Ciriolo, Mr; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/39234
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