The induction of cell death in human erythroleukemic cells ( K562) by sorbitol shows the typical apoptotic changes in ultrastructural morphology, including blebbing, chromatin condensation and nuclear membrane breakdown.. Using a cytofluorimetric approach, we found that sorbitol induced production of reactive oxygen species ( ROS) followed by DNA fragmentation in leukemic cells. In this study, we investigated effect of curcumin and rosmarinic acid on cell viability in three different cell lines: erythroleukemia K562, papillary NPA, and anaplastic ARO thyroid cancers. Curcumin was able to induce apoptosis in a concentration- and time dependent manner in three cell lines, while rosmarinic acid was less effective on this process. To examine this possibility in cellular system, this study evaluated the capacities of both compounds acting as antioxidant inhibiting sorbitolinduced apoptosis. K562, NPA and ARO cells were preincubated with 25 mu M rosmarinic acid to allow the uptake and then the cell lines were treated with 1 M sorbitol. Afterwards, the cells were subjected to agarose gel electrophoresis to assess the DNA fragmentation. In conclusion, the antioxidant activity of rosmarinic acid is able to inhibit sorbitol- induced apoptosis.

Sinibaldi Salimei, P., Marfe, G., Di Renzo, L., Di Stefano, C., Giganti, M.G., Filomeni, G., et al. (2007). The interference of rosmarinic acid in the DNA fragmentation induced by osmotic shock. FRONTIERS IN BIOSCIENCE, 12(4), 1308-1317 [10.2741/2149].

The interference of rosmarinic acid in the DNA fragmentation induced by osmotic shock

Sinibaldi Salimei, P;GIGANTI, MARIA GABRIELLA;FILOMENI, GIUSEPPE;CIRIOLO, MARIA ROSA
2007

Abstract

The induction of cell death in human erythroleukemic cells ( K562) by sorbitol shows the typical apoptotic changes in ultrastructural morphology, including blebbing, chromatin condensation and nuclear membrane breakdown.. Using a cytofluorimetric approach, we found that sorbitol induced production of reactive oxygen species ( ROS) followed by DNA fragmentation in leukemic cells. In this study, we investigated effect of curcumin and rosmarinic acid on cell viability in three different cell lines: erythroleukemia K562, papillary NPA, and anaplastic ARO thyroid cancers. Curcumin was able to induce apoptosis in a concentration- and time dependent manner in three cell lines, while rosmarinic acid was less effective on this process. To examine this possibility in cellular system, this study evaluated the capacities of both compounds acting as antioxidant inhibiting sorbitolinduced apoptosis. K562, NPA and ARO cells were preincubated with 25 mu M rosmarinic acid to allow the uptake and then the cell lines were treated with 1 M sorbitol. Afterwards, the cells were subjected to agarose gel electrophoresis to assess the DNA fragmentation. In conclusion, the antioxidant activity of rosmarinic acid is able to inhibit sorbitol- induced apoptosis.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
antioxidant; apoptosis regulatory protein; cinnamic acid derivative; curcumin; depside; DNA fragment; rosmarinic acid; sorbitol; apoptosis; article; cell strain K 562; drug antagonism; drug effect; genetics; human; metabolism; osmotic pressure; tumor cell line; Antioxidants; Apoptosis; Apoptosis Regulatory Proteins; Cell Line, Tumor; Cinnamates; Curcumin; Depsides; DNA Fragmentation; Humans; K562 Cells; Osmotic Pressure; Sorbitol
Sinibaldi Salimei, P., Marfe, G., Di Renzo, L., Di Stefano, C., Giganti, M.G., Filomeni, G., et al. (2007). The interference of rosmarinic acid in the DNA fragmentation induced by osmotic shock. FRONTIERS IN BIOSCIENCE, 12(4), 1308-1317 [10.2741/2149].
Sinibaldi Salimei, P; Marfe, G; Di Renzo, L; Di Stefano, C; Giganti, Mg; Filomeni, G; Ciriolo, Mr
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/35868
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