: In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models of colorectal cancer (CRC) crosslinking hyaluronic acid and gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose and 2'-Fucosylgalactose. The crosslinking, performed exploiting azide functionalized gelatin and hyaluronic acid and 4arm-PEG-dibenzocyclooctyne, resulted in biocompatible hydrogels that were 3D bioprinted with commercial CRC cells HT-29 and patient derived CRC tumoroids. The glycosylated hydrogels showed good 3D printability, biocompatibility and stability over the time. SEM and synchrotron radiation SAXS/WAXS analysis revealed the influence of glycosylation in the construct morphology, whereas MALDI-MS imaging showed that protein profiles of tumoroid cells vary with glycosylation, indicating that sialylation and fucosylation of ECM proteins induce diverse alterations to the proteome of the tumoroid and surrounding cells.

Cadamuro, F., Marongiu, L., Marino, M., Tamini, N., Nespoli, L., Zucchini, N., et al. (2023). 3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans. CARBOHYDRATE POLYMERS, 302 [10.1016/j.carbpol.2022.120395].

3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans

Marino M.;
2023-01-01

Abstract

: In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models of colorectal cancer (CRC) crosslinking hyaluronic acid and gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose and 2'-Fucosylgalactose. The crosslinking, performed exploiting azide functionalized gelatin and hyaluronic acid and 4arm-PEG-dibenzocyclooctyne, resulted in biocompatible hydrogels that were 3D bioprinted with commercial CRC cells HT-29 and patient derived CRC tumoroids. The glycosylated hydrogels showed good 3D printability, biocompatibility and stability over the time. SEM and synchrotron radiation SAXS/WAXS analysis revealed the influence of glycosylation in the construct morphology, whereas MALDI-MS imaging showed that protein profiles of tumoroid cells vary with glycosylation, indicating that sialylation and fucosylation of ECM proteins induce diverse alterations to the proteome of the tumoroid and surrounding cells.
2023
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ICAR/08 - SCIENZA DELLE COSTRUZIONI
English
3D bioprinting
Bioresponsive hydrogels
Colorectal cancer models
Glycosignature
Hyaluronic acid
Cadamuro, F., Marongiu, L., Marino, M., Tamini, N., Nespoli, L., Zucchini, N., et al. (2023). 3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans. CARBOHYDRATE POLYMERS, 302 [10.1016/j.carbpol.2022.120395].
Cadamuro, F; Marongiu, L; Marino, M; Tamini, N; Nespoli, L; Zucchini, N; Terzi, A; Altamura, D; Gao, Z; Giannini, C; Bindi, G; Smith, A; Magni, F; Bertini, S; Granucci, F; Nicotra, F; Russo, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/329605
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