In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.

Klionsky, D.j., Abdelmohsen, K., Abe, A., Abedin, M.j., Abeliovich, H., Acevedo Arozena, A., et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). AUTOPHAGY, 12(1), 1-222 [10.1080/15548627.2015.1100356].

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Amelio, Ivano
Membro del Collaboration Group
;
Antonioli, Manuela
Membro del Collaboration Group
;
Aquilano, Katia
Membro del Collaboration Group
;
Bernassola, Francesca
Membro del Collaboration Group
;
Bordi, Matteo
Membro del Collaboration Group
;
Campanella, Michelangelo
Membro del Collaboration Group
;
Campello, Silvia
Membro del Collaboration Group
;
Cecconi, Francesco
Membro del Collaboration Group
;
Cianfanelli, Valentina
Membro del Collaboration Group
;
Ciriolo, Maria Rosa
Membro del Collaboration Group
;
Corazzari, Marco
Membro del Collaboration Group
;
De Zio, Daniela
Membro del Collaboration Group
;
Di Bartolomeo, Sabrina
Membro del Collaboration Group
;
Di Daniele, Nicola
Membro del Collaboration Group
;
Federici, Massimo
Membro del Collaboration Group
;
Filomeni, Giuseppe
Membro del Collaboration Group
;
Hernandez, Carlos
Membro del Collaboration Group
;
Lettieri Barbato, Daniele
Membro del Collaboration Group
;
Marfe, Gabriella
Membro del Collaboration Group
;
Melino, Sonia
Membro del Collaboration Group
;
Menghini, Rossella
Membro del Collaboration Group
;
Palumbo, Camilla
Membro del Collaboration Group
;
Piacentini, Mauro
Membro del Collaboration Group
;
Radogna, Flavia
Membro del Collaboration Group
;
Schiavi, Alfonso
Membro del Collaboration Group
;
Ventura, Natascia
Membro del Collaboration Group
;
Viscomi, Maria Teresa
Membro del Collaboration Group
;
Wang, Jing
Membro del Collaboration Group
;
Yu, Li
Membro del Collaboration Group
;
Zhang, Li
Membro del Collaboration Group
;
2016-01-01

Abstract

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.
2016
Pubblicato
Rilevanza internazionale
Recensione
Esperti anonimi
Settore BIO/06 - ANATOMIA COMPARATA E CITOLOGIA
English
LC3; autolysosome; autophagosome; chaperone-mediated autophagy; flux; lysosome; macroautophagy; phagophore; stress; vacuole
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835977/
Klionsky, D.j., Abdelmohsen, K., Abe, A., Abedin, M.j., Abeliovich, H., Acevedo Arozena, A., et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). AUTOPHAGY, 12(1), 1-222 [10.1080/15548627.2015.1100356].
Klionsky, Dj; Abdelmohsen, K; Abe, A; Abedin, Mj; Abeliovich, H; Acevedo Arozena, A; Adachi, H; Adams, Cm; Adams, Pd; Adeli, K; Adhihetty, Pj; Adler, ...espandi
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/314344
Citazioni
  • ???jsp.display-item.citation.pmc??? 2831
  • Scopus 4385
  • ???jsp.display-item.citation.isi??? 3953
social impact