Epilithic phototrophic biofilms develop inside Roman Necropolis and Catacombs on rock surfaces exposed to artificial light sources and are composed by a microbial consortium dominated by cyanobacteria. In this work, six non-axenic cultures of Leptolyngbya sp. strains isolated frombiofilms from different Romanhypogea and maintained in cultures from 11 to 20 years were analysed along with their associated bacteria isolated in culture. The employment of PCR-fingerprinting techniques, using HIP1 and ERIC derived primers, allowed the clustering in three groups of the six Leptolyngbya strains and the typing of their isolated bacteria. The bacterial fingerprinting patterns were in agreement with the 16S rRNA gene sequencing and showed the presence in Leptolyngbya isolates of Pseudomonas, Stenotrophomonas, Agrobacterium and Bacillus representatives that were detected also in biofilms sampled from catacombs.

Bruno, L., Billi, D., Albertano, P.b., Urzi, C. (2006). PCR-genotyping of cyanobacteria from Roman hypogea biofilms and their associated bacteria. In 1th International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld-2005) "Fostering Cross-disciplinary Applied Research in Microbiology and Microbial Biotechnology".

PCR-genotyping of cyanobacteria from Roman hypogea biofilms and their associated bacteria

BRUNO, LAURA;BILLI, DANIELA;ALBERTANO, PATRIZIA BEATRICE;
2006-01-01

Abstract

Epilithic phototrophic biofilms develop inside Roman Necropolis and Catacombs on rock surfaces exposed to artificial light sources and are composed by a microbial consortium dominated by cyanobacteria. In this work, six non-axenic cultures of Leptolyngbya sp. strains isolated frombiofilms from different Romanhypogea and maintained in cultures from 11 to 20 years were analysed along with their associated bacteria isolated in culture. The employment of PCR-fingerprinting techniques, using HIP1 and ERIC derived primers, allowed the clustering in three groups of the six Leptolyngbya strains and the typing of their isolated bacteria. The bacterial fingerprinting patterns were in agreement with the 16S rRNA gene sequencing and showed the presence in Leptolyngbya isolates of Pseudomonas, Stenotrophomonas, Agrobacterium and Bacillus representatives that were detected also in biofilms sampled from catacombs.
(BioMicroWorld-2005) "Fostering Cross-disciplinary Applied Research in Microbiology and Microbial Biotechnology"
Badajoz, SPAIN
MAR 15-18, 2005
Rilevanza internazionale
contributo
2005
2006
Settore BIO/01 - BOTANICA GENERALE
English
Associated bacteria; Biodeterioration; Cyanobacteria; Epilithic biofilms; Genome PCR-fingerprinting; Leptolyngbya
Intervento a convegno
Bruno, L., Billi, D., Albertano, P.b., Urzi, C. (2006). PCR-genotyping of cyanobacteria from Roman hypogea biofilms and their associated bacteria. In 1th International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld-2005) "Fostering Cross-disciplinary Applied Research in Microbiology and Microbial Biotechnology".
Bruno, L; Billi, D; Albertano, Pb; Urzi, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/30647
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