Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the "hard-core" reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte-macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.
Alteri, C., Piermatteo, L., Silberstein, F.c., Svicher, V., Perno, C.f. (2022). Human monocyte-derived macrophages (MDM): model 1 (GM-CSF). In HIV reservoirs (pp. 91-96). Springer Science+Business Media, LLC, part of Springer Nature [10.1007/978-1-0716-1871-4_8].
Human monocyte-derived macrophages (MDM): model 1 (GM-CSF)
Piermatteo, Lorenzo;Silberstein, Francesca Ceccherini;Svicher, Valentina;Perno, Carlo Federico
2022-01-01
Abstract
Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the "hard-core" reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte-macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
