We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the "translator," that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control protein-protein communication in artificial regulatory networks.

Ranallo, S., Sorrentino, D., Delibato, E., Ercolani, G., Plaxco, K.w., Ricci, F. (2022). Protein–protein communication mediated by an antibody-responsive DNA nanodevice. ANGEWANDTE CHEMIE, 61(12) [10.1002/anie.202115680].

Protein–protein communication mediated by an antibody-responsive DNA nanodevice

Sorrentino D.;Ercolani G.;Ricci F.
2022-01-01

Abstract

We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the "translator," that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control protein-protein communication in artificial regulatory networks.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01
Settore CHIM/06
English
Aptamers
DNA Nanotechnology
Protein-Protein Communication
Strand Displacement Reaction
Synthetic Biology
https://doi.org/10.33774/chemrxiv-2021-zz8q0
Ranallo, S., Sorrentino, D., Delibato, E., Ercolani, G., Plaxco, K.w., Ricci, F. (2022). Protein–protein communication mediated by an antibody-responsive DNA nanodevice. ANGEWANDTE CHEMIE, 61(12) [10.1002/anie.202115680].
Ranallo, S; Sorrentino, D; Delibato, E; Ercolani, G; Plaxco, Kw; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/293011
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