Background & Aim: In Crohn's disease (CD) mucosa, lamina propria T lymphocytes (LPL) express high levels of Fas but are resistant to Fas-mediated apoptosis. The molecular mechanism underlying the resistance of CD LPL to FAS-mediated apoptosis still remains unclear. The aim of present study was to examine the expression and the role of FLICE-inhibitory protein (Flip), an inhibitor of the Fas signaling pathway, in CD. Materials and Methods: Whole intestinal mucosa, lamina propria mononuclear cells (LPMC) and LP CD3+T lymphocytes (LPL) were obtained from CD and ulcerative colitis (UC) patients and normal controls and analyzed for the expression of Flip by Western blotting, using a monoclonal antibody that recognizes both the full-length and short form of Flip. Results: The full-length form of Flip was constitutively detected in all samples, but its expression was higher in whole biopsies and mucosal cells from CD patients in comparison to UC and normal controls. In addition, CD samples exhibit higher levels of the short form of Flip, which negatively regulates the activity of initiator caspases. Discussion: Data suggest that high Flip expression contributes to confer resistance of mucosal T cells to Fas-mediated apoptosis in CD.

Monteleone I., F.L. (2004). Enhanced expression of the Fas pathway inhibitor, Flip, in the mucosa of patients with Crohn's disease. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 8(5), 192.

Enhanced expression of the Fas pathway inhibitor, Flip, in the mucosa of patients with Crohn's disease

MONTELEONE, IVAN;BIANCONE, LIVIA;PALLONE, FRANCESCO;MONTELEONE, GIOVANNI
2004

Abstract

Background & Aim: In Crohn's disease (CD) mucosa, lamina propria T lymphocytes (LPL) express high levels of Fas but are resistant to Fas-mediated apoptosis. The molecular mechanism underlying the resistance of CD LPL to FAS-mediated apoptosis still remains unclear. The aim of present study was to examine the expression and the role of FLICE-inhibitory protein (Flip), an inhibitor of the Fas signaling pathway, in CD. Materials and Methods: Whole intestinal mucosa, lamina propria mononuclear cells (LPMC) and LP CD3+T lymphocytes (LPL) were obtained from CD and ulcerative colitis (UC) patients and normal controls and analyzed for the expression of Flip by Western blotting, using a monoclonal antibody that recognizes both the full-length and short form of Flip. Results: The full-length form of Flip was constitutively detected in all samples, but its expression was higher in whole biopsies and mucosal cells from CD patients in comparison to UC and normal controls. In addition, CD samples exhibit higher levels of the short form of Flip, which negatively regulates the activity of initiator caspases. Discussion: Data suggest that high Flip expression contributes to confer resistance of mucosal T cells to Fas-mediated apoptosis in CD.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/12 - Gastroenterologia
English
caspase; CD3 antigen; Fas antigen; FLICE inhibitory protein; monoclonal antibody; apoptosis; article; controlled study; Crohn disease; data analysis; enzyme regulation; gene expression; human; human tissue; intestine biopsy; intestine mucosa; lamina propria; mononuclear cell; mucosa cell; sample; signal transduction; T lymphocyte; ulcerative colitis; Western blotting
Monteleone I., F.L. (2004). Enhanced expression of the Fas pathway inhibitor, Flip, in the mucosa of patients with Crohn's disease. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 8(5), 192.
Monteleone, I; Franchi, L; Biancone, L; Pallone, F; Monteleone, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/28780
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