Frequency dependent functional brain reorganization in anesthesia is specific to drug concentration

The differential effects of general anesthesia on brain activity in terms of drug selection, concentration and combination remain to be elucidated. Using fMRI, it has been shown that increasing doses of sevoflurane is associated with progressive breakdown in brain functional connectivity, while EEG studies have shown that higher activity in the delta band is associated with unconsciousness. Despite these promising results, the band- specific neural substrates of brain changes which occur during sevoflurane anesthesia have not yet been investigated. To this end, we employ high-density EEG-based brain connectivity estimates and graph theoretical analysis in a protocol of progressive sevoflurane administration (conditions: baseline, 1.1%, 2.1%, 2.8%, recovery), both at a global (whole-brain) and at a local (sensor-specific) level in 12 healthy subjects (7 males, mean age 25 ± 4.7 years). We show a statistically significant dependence of global strength, clustering coefficient and efficiency on sevoflurane concentration in the slow delta, beta 1 and beta 2 bands. Interestingly, high and low-frequency bands behaved in an opposite manner as a function of condition. We also found significant band∗condition interactive effects in clustering coefficient, efficiency and strength both on local and global scales.