This paper reports a system for the quantitative assessment of dielectrophoretic (DEP) focusing of particles flowing in a microfluidic impedance cytometer. The cytometer performs an electrical measurement of particle trajectories lateral positions before and after a DEP focusing region. Lateral positions are measured using a non-standard electrode configuration yielding an asymmetric bipolar Gaussian signal. The peak amplitude relative difference Δ of the signal (introduced in previous work to compensate for positional dependence effects) correlates with particle position. The system was validated by analyzing the focusing of polystyrene beads for different DEP field intensities, obtaining different degrees of focusing.
Reale, R., De Ninno, A., Businaro, L., Bisegna, P., Caselli, F. (2018). Electrical quantitative assessment of dielectrophoretic focusing in a microchannel. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp.1384-1386). Chemical and Biological Microsystems Society.
Electrical quantitative assessment of dielectrophoretic focusing in a microchannel
Reale R.;Bisegna P.;Caselli F.
2018-01-01
Abstract
This paper reports a system for the quantitative assessment of dielectrophoretic (DEP) focusing of particles flowing in a microfluidic impedance cytometer. The cytometer performs an electrical measurement of particle trajectories lateral positions before and after a DEP focusing region. Lateral positions are measured using a non-standard electrode configuration yielding an asymmetric bipolar Gaussian signal. The peak amplitude relative difference Δ of the signal (introduced in previous work to compensate for positional dependence effects) correlates with particle position. The system was validated by analyzing the focusing of polystyrene beads for different DEP field intensities, obtaining different degrees of focusing.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
