Klebsiella pneumoniae carbapenemases (KPC)-producing Klebsiella pneumoniae represents a major clinical problem given the lack of available treatment options. In this work we have characterized one bacteriophage, named vB_Kpn_F48, with a lytic activity against KPC-positive Klebsiella pneumoniae (KPC-Kp) of Sequence Type (ST) 101. Transmission electron microscopy (TEM), burst size, host range and sensitivity to temperature and pH were used to characterize phage vB_Kpn_F48. Molecular characterization through High Throughput DNA Sequencing and direct sequencing by Sanger method was employed to define the phage genomic sequence, while genome annotation was performed by RAST analysis. Bacteriophage vB_Kpn_F48 was classified as a member of Myoviridae, order Caudovirales, on the basis of TEM analysis. Physiological characterization demonstrated that vB_Kpn_F48 is highly stable to both temperature and pH variations. A total of 283 possible coding regions have been detected by RAST analysis and a hypothetical function was assigned to 120 (42.4%). The obtained results suggest that vB_Kpn_F48 could represent a valid alternative therapeutic agent. In addition, the phage exhibits a number of properties indicative of a potential utility in phage therapy cocktails.

Ciacci, N., D’Andrea, M.m., Marmo, P., Demattè, E., Vannuccini, E., Lupetti, P., et al. (2017). Isolation and characterisation of vB_Kpn_F48, a novel bacteriophage for Klebsiella pneumoniae. In Abstract Book Congresso.

Isolation and characterisation of vB_Kpn_F48, a novel bacteriophage for Klebsiella pneumoniae

CIACCI N.;D’ANDREA M. M.;MARMO P.;THALLER Maria Cristina
2017-09-17

Abstract

Klebsiella pneumoniae carbapenemases (KPC)-producing Klebsiella pneumoniae represents a major clinical problem given the lack of available treatment options. In this work we have characterized one bacteriophage, named vB_Kpn_F48, with a lytic activity against KPC-positive Klebsiella pneumoniae (KPC-Kp) of Sequence Type (ST) 101. Transmission electron microscopy (TEM), burst size, host range and sensitivity to temperature and pH were used to characterize phage vB_Kpn_F48. Molecular characterization through High Throughput DNA Sequencing and direct sequencing by Sanger method was employed to define the phage genomic sequence, while genome annotation was performed by RAST analysis. Bacteriophage vB_Kpn_F48 was classified as a member of Myoviridae, order Caudovirales, on the basis of TEM analysis. Physiological characterization demonstrated that vB_Kpn_F48 is highly stable to both temperature and pH variations. A total of 283 possible coding regions have been detected by RAST analysis and a hypothetical function was assigned to 120 (42.4%). The obtained results suggest that vB_Kpn_F48 could represent a valid alternative therapeutic agent. In addition, the phage exhibits a number of properties indicative of a potential utility in phage therapy cocktails.
XXXII Congresso SIMGBM
Palermo
2017
XXXII
Rilevanza internazionale
contributo
17-set-2017
Settore BIO/19 - MICROBIOLOGIA GENERALE
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Intervento a convegno
Ciacci, N., D’Andrea, M.m., Marmo, P., Demattè, E., Vannuccini, E., Lupetti, P., et al. (2017). Isolation and characterisation of vB_Kpn_F48, a novel bacteriophage for Klebsiella pneumoniae. In Abstract Book Congresso.
Ciacci, N; D’Andrea, Mm; Marmo, P; Demattè, E; Vannuccini, E; Lupetti, P; Rossolini, Gm; Thaller, Mc
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/249721
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