Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

This work presents a portable lab-on-chip system, based on thin lm electronic devices and an all-glass micro uidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The mi- cro uidic network is patterned, through wet etching, in a 1.1mm-thick glass substrate that is subsequently bonded to a 0.5mm-thick glass sub- strate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosen- sors, the micro uidic network and the electronic boards reading out the photodiodes' current are enclosed in a small metallic box (10x8x15cm3) in order to ensure shielding from electromagnetic interferences. Prelimi- nary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hy- drogen peroxide. Limits of detection and quantication equal to 18 and 60 M, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.