Background and Aims In ulcerative colitis [UC], mucosal damage occurs in areas that are infiltrated with neutrophils. The antimicrobial function of neutrophils relies in part on the formation of extracellular web-like structures, named neutrophil extracellular traps [NETs]. The formation and/or clearance of aberrant NETs have been associated with several immune diseases. Here we investigated the role of NETs in UC-related inflammation.Methods The expression of NET-associated proteins was evaluated in colonic biopsies of patients with Crohn's disease [CD], UC and in normal controls [NC] by Western blotting, immunofluorescence and immunohistochemistry. Colonic biopsies of UC patients were analysed before and after anti-tumour necrosis factor [anti-TNF-] treatment. The capacity of neutrophils to produce NETs upon activation was tested in vitro. UC lamina propria mononuclear cells [LPMCs] were cultured with NETs in the presence or absence of an extracellular signal-regulated kinase-1/2 [ERK1/2] inhibitor and inflammatory cytokine induction was assessed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. We also characterized the contribution of NETs in dextran sodium sulfate [DSS]-induced colitis.Results NET-associated proteins were over-expressed in inflamed colon of UC patients as compared to CD patients and NC. Circulating neutrophils of UC patients produced NETs in response to TNF- stimulation, and reduced expression of NET-related proteins and diminished NET formation were seen in patients receiving successful treatment with anti-TNF-. Treatment of UC LPMCs with NETs activated ERK1/2, thus enhancing TNF- and interleukin-1 [IL-1] production. NETs were induced in mice with DSS-colitis and in vivo inhibition of NET release attenuated colitis.Conclusions Our data show that NET release occurs in UC and suggest a role for NETs in sustaining mucosal inflammation in this disorder.

Dinallo, V., Marafini, I., Fusco, D.d., Laudisi, F., Franze, E., Grazia, A.d., et al. (2019). Neutrophil extracellulartraps sustain inflammatory signals in ulcerative colitis. JOURNAL OF CROHN'S AND COLITIS, 13(6), 772-784 [10.1093/ecco-jcc/jjy215].

Neutrophil extracellulartraps sustain inflammatory signals in ulcerative colitis

Dinallo V.;Marafini I.;Stolfi C.;Monteleone I.;Monteleone G.
2019-02-03

Abstract

Background and Aims In ulcerative colitis [UC], mucosal damage occurs in areas that are infiltrated with neutrophils. The antimicrobial function of neutrophils relies in part on the formation of extracellular web-like structures, named neutrophil extracellular traps [NETs]. The formation and/or clearance of aberrant NETs have been associated with several immune diseases. Here we investigated the role of NETs in UC-related inflammation.Methods The expression of NET-associated proteins was evaluated in colonic biopsies of patients with Crohn's disease [CD], UC and in normal controls [NC] by Western blotting, immunofluorescence and immunohistochemistry. Colonic biopsies of UC patients were analysed before and after anti-tumour necrosis factor [anti-TNF-] treatment. The capacity of neutrophils to produce NETs upon activation was tested in vitro. UC lamina propria mononuclear cells [LPMCs] were cultured with NETs in the presence or absence of an extracellular signal-regulated kinase-1/2 [ERK1/2] inhibitor and inflammatory cytokine induction was assessed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. We also characterized the contribution of NETs in dextran sodium sulfate [DSS]-induced colitis.Results NET-associated proteins were over-expressed in inflamed colon of UC patients as compared to CD patients and NC. Circulating neutrophils of UC patients produced NETs in response to TNF- stimulation, and reduced expression of NET-related proteins and diminished NET formation were seen in patients receiving successful treatment with anti-TNF-. Treatment of UC LPMCs with NETs activated ERK1/2, thus enhancing TNF- and interleukin-1 [IL-1] production. NETs were induced in mice with DSS-colitis and in vivo inhibition of NET release attenuated colitis.Conclusions Our data show that NET release occurs in UC and suggest a role for NETs in sustaining mucosal inflammation in this disorder.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/12
Settore BIO/12
English
Con Impact Factor ISI
NETosis; PAD4; inflammatory bowel disease; neutrophils; ulcerative colitis; Animals; Colitis, Ulcerative; Colon; Disease Models, Animal; Extracellular Traps; Female; Fluorescent Antibody Technique; Humans; Inflammation; Interleukin-1beta; Intestinal Mucosa; MAP Kinase Signaling System; Mice; Mice, Inbred BALB C; Real-Time Polymerase Chain Reaction; Tumor Necrosis Factor-alpha
Dinallo, V., Marafini, I., Fusco, D.d., Laudisi, F., Franze, E., Grazia, A.d., et al. (2019). Neutrophil extracellulartraps sustain inflammatory signals in ulcerative colitis. JOURNAL OF CROHN'S AND COLITIS, 13(6), 772-784 [10.1093/ecco-jcc/jjy215].
Dinallo, V; Marafini, I; Fusco, Dd; Laudisi, F; Franze, E; Grazia, Ad; Figliuzzi, Mm; Caprioli, F; Stolfi, C; Monteleone, I; Monteleone, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/245626
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