Intrahepatic total HBV DNA (it-HBV DNA) level might reflect the size of virus reservoir and correlate with the histological status of the liver. To quantitate it-HBV DNA in a series of 70 liver biopsies obtained from hepatitis B chronic patients, a modified version of the -COBAS (R) Ampliprep/COBAS (R) TaqMan HBV test v2.0 was used for this purpose. The linearity and reproducibility of the modified protocol was tested by quantifying serial dilutions of a full-length HBV containing plasmid and it-HBV DNA from a reference patient. A good linear trend between the expected values and those generated by the assay was observed at different concentrations of both plasmid and reference patient (R-2 = 0.994 and 0.962, respectively). Differences between the values obtained in two independent runs were >= 0.3 log IU for the plasmid and <= 0.6 log IU/mg for the reference patient, showing a high inter-run reproducibility. In the 70 liver biopsies, it-HBV DNA level ranged from 1.4 to 5.4 log IU/mg, with a good linearity and reproducibility between the values obtained in two runs [R-2 = 0.981; median (IQR) difference of it-HBV DNA 0.05 (0.02-0.09) IU/mg]. The modified -COBAS (R) Ampliprep/COBAS (R) TaqMan HBV test v2.0 allows an accurate quantitation of it-HBV DNA. Its determination may have prognostic value and may be a useful tool for the new therapeutic strategies aimed at eradicating the HBV infection.

Salpini, R., Piermatteo, L., Gill, U., Battisti, A., Stazi, F., Guenci, T., et al. (2017). Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS® Ampliprep/COBAS®TaqMan HBV test v2.0. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 206(4), 295-299 [10.1007/s00430-017-0504-3].

Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS® Ampliprep/COBAS®TaqMan HBV test v2.0

Salpini R.;Piermatteo L.;Perno C. F.;Svicher V.;
2017-01-01

Abstract

Intrahepatic total HBV DNA (it-HBV DNA) level might reflect the size of virus reservoir and correlate with the histological status of the liver. To quantitate it-HBV DNA in a series of 70 liver biopsies obtained from hepatitis B chronic patients, a modified version of the -COBAS (R) Ampliprep/COBAS (R) TaqMan HBV test v2.0 was used for this purpose. The linearity and reproducibility of the modified protocol was tested by quantifying serial dilutions of a full-length HBV containing plasmid and it-HBV DNA from a reference patient. A good linear trend between the expected values and those generated by the assay was observed at different concentrations of both plasmid and reference patient (R-2 = 0.994 and 0.962, respectively). Differences between the values obtained in two independent runs were >= 0.3 log IU for the plasmid and <= 0.6 log IU/mg for the reference patient, showing a high inter-run reproducibility. In the 70 liver biopsies, it-HBV DNA level ranged from 1.4 to 5.4 log IU/mg, with a good linearity and reproducibility between the values obtained in two runs [R-2 = 0.981; median (IQR) difference of it-HBV DNA 0.05 (0.02-0.09) IU/mg]. The modified -COBAS (R) Ampliprep/COBAS (R) TaqMan HBV test v2.0 allows an accurate quantitation of it-HBV DNA. Its determination may have prognostic value and may be a useful tool for the new therapeutic strategies aimed at eradicating the HBV infection.
2017
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
COBAS® TaqMan HBV test, v2.0; HBV DNA level; HBV reservoir; Intrahepatic HBV DNA; Adult; Biopsy; DNA, Viral; Female; Hepatitis B virus; Hepatitis B, Chronic; Humans; Liver; Male; Reproducibility of Results; Viral Load
Salpini, R., Piermatteo, L., Gill, U., Battisti, A., Stazi, F., Guenci, T., et al. (2017). Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS® Ampliprep/COBAS®TaqMan HBV test v2.0. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 206(4), 295-299 [10.1007/s00430-017-0504-3].
Salpini, R; Piermatteo, L; Gill, U; Battisti, A; Stazi, F; Guenci, T; Giannella, S; Serafini, V; Kennedy, Ptf; Perno, Cf; Svicher, V; Ciotti, M...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/238778
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