In this study, we found that production of both reactive oxygen ( ROS) and nitrogen ( RNS) species is a very early event related to treatment with hyperosmotic concentration of sorbitol. The production of nitric oxide ( NO) was paralleled by the increase of the mRNA and protein level of the inducible form of the nitric oxide synthase ( iNOS). ROS and RNS enhancement, process concomitant to the failure of mitochondrial trans-membrane potential (Delta Psi), was necessary for the induction of apoptosis as demonstrated by the protection against sorbitol-mediated toxicity observed after treatment with ROS scavengers or NOS inhibitors. The synergistic action of ROS and RNS was finally demonstrated by pre-treatment with rosmarinic acid that, by powerfully buffering both these species, prevents impairment of Delta Psi and cell death. Overall results suggest that the occurrence of apoptosis upon sorbitol treatment is an event mediated by oxidative/nitrosative stress rather than a canonical hyperosmotic shock.

Aquilano, K., Filomeni, G., Di Renzo, L., Di Vito, M., Di Stefano, C., Salimei, P.S., et al. (2007). Reactive oxygen and nitrogen species are involved in sorbitol-induced apoptosis of human erithroleukaemia cells K562. FREE RADICAL RESEARCH, 41(4), 452-460 [10.1080/10715760601134459].

Reactive oxygen and nitrogen species are involved in sorbitol-induced apoptosis of human erithroleukaemia cells K562

AQUILANO, KATIA;Filomeni, G;CIRIOLO, MARIA ROSA;
2007

Abstract

In this study, we found that production of both reactive oxygen ( ROS) and nitrogen ( RNS) species is a very early event related to treatment with hyperosmotic concentration of sorbitol. The production of nitric oxide ( NO) was paralleled by the increase of the mRNA and protein level of the inducible form of the nitric oxide synthase ( iNOS). ROS and RNS enhancement, process concomitant to the failure of mitochondrial trans-membrane potential (Delta Psi), was necessary for the induction of apoptosis as demonstrated by the protection against sorbitol-mediated toxicity observed after treatment with ROS scavengers or NOS inhibitors. The synergistic action of ROS and RNS was finally demonstrated by pre-treatment with rosmarinic acid that, by powerfully buffering both these species, prevents impairment of Delta Psi and cell death. Overall results suggest that the occurrence of apoptosis upon sorbitol treatment is an event mediated by oxidative/nitrosative stress rather than a canonical hyperosmotic shock.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
English
Con Impact Factor ISI
Apoptosis; Mitochondrial transmembrane potential; RNS; ROS; Rosmarinic acid; Sorbitol
Aquilano, K., Filomeni, G., Di Renzo, L., Di Vito, M., Di Stefano, C., Salimei, P.S., et al. (2007). Reactive oxygen and nitrogen species are involved in sorbitol-induced apoptosis of human erithroleukaemia cells K562. FREE RADICAL RESEARCH, 41(4), 452-460 [10.1080/10715760601134459].
Aquilano, K; Filomeni, G; Di Renzo, L; Di Vito, M; Di Stefano, C; Salimei, P; Ciriolo, Mr; Marfe, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/23675
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