Smartphones are developing into all-purposes devices. In the present work, the employment/application of smartphones as medical devices in home care and point-of-care (POC) diagnostics are investigated in the analysis of Lateral Flow Assays (LFA). A smartphone-based LFA reader was developed for the quantitative analysis of D-Dimer - a biomarker indicating e.g. thrombotic event or danger of embolism.The proof-of-concept has been shown with multiple smartphones in establishing:Optimal dimensions of the LFA cell of 72.11mm distance of smartphone to D-Dimer test leading to a coefficients of variances (CV) between 0.8% and 4.2%Inter-device investigations: CVs around 13.5%; a limit of detection (LOD) of 100ng/ml (DDU) D-DimerInter-smartphone investigations: CV about 16%, a limit of detection (LOD) at 66.4ng/ml (DDU)Calibrations: CV and LOD of three smartphones are comparable to the commercial available LFA readerFurther development to put the multiple smartphone-based LFA reader on the market.
Schneider, S., Selig, M., Keil, V., Lehmann, M., Foitzik, A.h., Casalboni, M., et al. (2018). Development of a smartphone based reader for the quantitative analysis of lateral flow assays. ??????? it.cilea.surplus.oa.citation.tipologie.CitationProceedings.prensentedAt ??????? 10th International Conference on Processing and Manufacturing of Advanced Materials, 2018; Paris; France; 9 July 2018 through 13 July 2018; Code 223589 [10.4028/www.scientific.net/MSF.941.2522].
Development of a smartphone based reader for the quantitative analysis of lateral flow assays
Schneider S.;Casalboni M.;Richetta M.
2018-06-01
Abstract
Smartphones are developing into all-purposes devices. In the present work, the employment/application of smartphones as medical devices in home care and point-of-care (POC) diagnostics are investigated in the analysis of Lateral Flow Assays (LFA). A smartphone-based LFA reader was developed for the quantitative analysis of D-Dimer - a biomarker indicating e.g. thrombotic event or danger of embolism.The proof-of-concept has been shown with multiple smartphones in establishing:Optimal dimensions of the LFA cell of 72.11mm distance of smartphone to D-Dimer test leading to a coefficients of variances (CV) between 0.8% and 4.2%Inter-device investigations: CVs around 13.5%; a limit of detection (LOD) of 100ng/ml (DDU) D-DimerInter-smartphone investigations: CV about 16%, a limit of detection (LOD) at 66.4ng/ml (DDU)Calibrations: CV and LOD of three smartphones are comparable to the commercial available LFA readerFurther development to put the multiple smartphone-based LFA reader on the market.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
