The membrane-substrate interactions have a topological valence and represent a level of information exchange between the cell and the extra-cellular matrix and/or between cells. The interactions can vary with boundary conditions and can be altered by varying the chemical and/or physical properties of the substrate. The alteration can presumably result in differentiation or specialization of the cells, but this fundamental aspect must still be fully understood. In such a frame, we investigated the levels of transcriptional coactivators YAP/TAZ throughout C2C12 differentiation on standard two-dimensional substrates and on polyethylene glycol-fibrinogen three-dimensional microenvironment. In detail, we observed that the use of a three-dimensional matrix permits an earlier differentiation in muscular cells when compared to standard bidimensional substrates. On such a basis, we want to investigate the modulation of a more regular threedimensional pattern on cells proliferation response and we propose a matrix, generable with multiphoton absorption, with regular aligned channels in order to overcome the current limitation in muscle regeneration techniques, so a possible tool to improve the myofibers formation and alignment.

Errico, V., Molinaro, R., Gargioli, C., Ferranti, F., Dinescu, M., Cannata, S., et al. (2016). Cells microenvironment engineering multiphoton absorption for muscle regeneration optimization. ??????? it.cilea.surplus.oa.citation.tipologie.CitationProceedings.prensentedAt ??????? 9th International Conference on Biomedical Electronics and Devices, BIODEVICES 2016 - Part of 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016, ita.

Cells microenvironment engineering multiphoton absorption for muscle regeneration optimization

MOLINARO, RITA;GARGIOLI, CESARE;CANNATA, STEFANO;SAGGIO, GIOVANNI;RUFINI, STEFANO;DESIDERI, ALESSANDRO
2016

Abstract

The membrane-substrate interactions have a topological valence and represent a level of information exchange between the cell and the extra-cellular matrix and/or between cells. The interactions can vary with boundary conditions and can be altered by varying the chemical and/or physical properties of the substrate. The alteration can presumably result in differentiation or specialization of the cells, but this fundamental aspect must still be fully understood. In such a frame, we investigated the levels of transcriptional coactivators YAP/TAZ throughout C2C12 differentiation on standard two-dimensional substrates and on polyethylene glycol-fibrinogen three-dimensional microenvironment. In detail, we observed that the use of a three-dimensional matrix permits an earlier differentiation in muscular cells when compared to standard bidimensional substrates. On such a basis, we want to investigate the modulation of a more regular threedimensional pattern on cells proliferation response and we propose a matrix, generable with multiphoton absorption, with regular aligned channels in order to overcome the current limitation in muscle regeneration techniques, so a possible tool to improve the myofibers formation and alignment.
9th International Conference on Biomedical Electronics and Devices, BIODEVICES 2016 - Part of 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016
ita
2016
Rilevanza internazionale
Settore BIO/09
English
C2C12; Cell culture; Extracellular matrix; Hydrogel matrix; Multiphoton absorption; Muscle regeneration; Myofibers; Polyethylene glycol- fibrinogen;
Intervento a convegno
Errico, V., Molinaro, R., Gargioli, C., Ferranti, F., Dinescu, M., Cannata, S., et al. (2016). Cells microenvironment engineering multiphoton absorption for muscle regeneration optimization. ??????? it.cilea.surplus.oa.citation.tipologie.CitationProceedings.prensentedAt ??????? 9th International Conference on Biomedical Electronics and Devices, BIODEVICES 2016 - Part of 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016, ita.
Errico, V; Molinaro, R; Gargioli, C; Ferranti, F; Dinescu, M; Cannata, S; Saggio, G; Rufini, S; Desideri, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/181512
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