The establishment of neuronal polarity and axonal outgrowth are key processes affecting neuronal migration and synapse formation, their impairment likely leading to cognitive deficits. Here we have found that the apoptotic protease activating factor 1 (Apaf1), apart from its canonical role in apoptosis, plays an additional function in cortical neurons, where its deficiency specifically impairs axonal growth. Given the central role played by centrosomes and microtubules in the polarized extension of the axon, our data suggest that Apaf1-deletion affects axonal outgrowth through an impairment of centrosome organization. In line with this, centrosomal protein expression, as well as their centrosomal localization proved to be altered upon Apaf1-deletion. Strikingly, we also found that Apaf1-loss affects trans-Golgi components and leads to a robust activation of AMP-dependent protein kinase (AMPK), this confirming the stressful conditions induced by Apaf1-deficiency. Since AMPK hyper-phosphorylation is known to impair a proper axon elongation, our finding contributes to explain the effect of Apaf1-deficiency on axogenesis. We also discovered that the signaling pathways mediating axonal growth and involving glycogen synthase kinase-3β, liver kinase B1, and collapsing-response mediator protein-2 are altered in Apaf1-KO neurons. Overall, our results reveal a novel non-apoptotic role for Apaf1 in axonal outgrowth, suggesting that the neuronal phenotype due to Apaf1-deletion could not only be fully ascribed to apoptosis inhibition, but might also be the result of defects in axogenesis. The discovery of new molecules involved in axonal elongation has a clinical relevance since it might help to explain neurological abnormalities occurring during early brain development.
De Zio, D., Molinari, F., Rizza, S., Gatta, L., Ciotti, M.t., Salvatore, A.m., et al. (2015). Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. CELLULAR AND MOLECULAR LIFE SCIENCES, 72(21), 4173-4191 [10.1007/s00018-015-1927-x].
|Tipologia:||Articolo su rivista|
|Citazione:||De Zio, D., Molinari, F., Rizza, S., Gatta, L., Ciotti, M.t., Salvatore, A.m., et al. (2015). Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. CELLULAR AND MOLECULAR LIFE SCIENCES, 72(21), 4173-4191 [10.1007/s00018-015-1927-x].|
|Settore Scientifico Disciplinare:||Settore BIO/11|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00018-015-1927-x|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||nov-2015|
|Titolo:||Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth|
|Autori:||De Zio, D; Molinari, F; Rizza, S; Gatta, L; Ciotti, Mt; Salvatore, Am; Mathiassen, Sg; Cwetsch, Aw; Filomeni, G; Rosano, G; Ferraro, E|
|Appare nelle tipologie:||01 - Articolo su rivista|