Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Delta psi(m))-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.

Di Giacomo, G., Campello, S., Corrado, M., Di Giambattista, L., Cirotti, C., Filomeni, G., et al. (2015). Mature erythrocytes of Iguana iguana (Squamata, Iguanidae) possess functional mitochondria. PLOS ONE, 10(9) [10.1371/journal.pone.0136770].

Mature erythrocytes of Iguana iguana (Squamata, Iguanidae) possess functional mitochondria

CAMPELLO, SILVIA;CIROTTI, CLAUDIA;FILOMENI, GIUSEPPE;GENTILE, GABRIELE
2015-01-01

Abstract

Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Delta psi(m))-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.
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Rilevanza internazionale
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Esperti anonimi
Settore BIO/05
Settore BIO/10
English
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Di Giacomo, G., Campello, S., Corrado, M., Di Giambattista, L., Cirotti, C., Filomeni, G., et al. (2015). Mature erythrocytes of Iguana iguana (Squamata, Iguanidae) possess functional mitochondria. PLOS ONE, 10(9) [10.1371/journal.pone.0136770].
Di Giacomo, G; Campello, S; Corrado, M; Di Giambattista, L; Cirotti, C; Filomeni, G; Gentile, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/122770
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