Increasing evidence suggests that HLA-DRB1 alleles reduce or increase the risk of developing ulcerative colitis-associated colorectal carcinoma (CRC) tumors. However, the role of HLA-DRB1 locus on the susceptibility to develop CRC tumor, in the absence of a history of inflammatory bowel diseases (IBDs), is unclear. The aim of our study was to determine whether HLA-DRB1 alleles are associated with IBD-independent CRC tumor. HLA-DRB1 allele polymorphisms were identified by sequence-based typing method in 53 CRC patients and 57 sex- and age-matched healthy Caucasian controls. Pearson's chi-squared analysis with Yate's correction or Fisher's exact test with Bonferroni's correction, as appropriate, were used to compare the allele frequency (AF) differences of HLA-DRB1 in patients and controls. A total of 29 HLA-DRB1 alleles were recognized. A detailed study of these alleles allowed to identify DRB1*13:01 and DRB1*11:01 alleles that were significantly associated with an increased and reduced risk to develop CRC tumor, respectively. AF of DRB1*13:01, in CRC patients, was significantly higher than that of healthy controls, even following Bonferroni's correction (p = 0.029). In contrast, the presence of the DRB1*11:01 allele was negatively associated with CRC tumor as evidenced by the significantly lower AF in CRC patients than that of healthy controls (p = 0.005). However, following Bonferroni's correction, the AF of DRB*11:01 lost its statistical significance. These results suggest that HLA-DRB1*13:01 allele could be a potential marker for predicting genetic susceptibility to CRC tumor. In contrast, the protective role of DRB1*11:01 remains unclear.

Aureli, A., Canossi, A., Del Beato, T., Franceschilli, L., Buonomo, O.c., Papola, F., et al. (2015). HLA-DRB1∗13:01 allele in the genetic susceptibility to colorectal carcinoma. INTERNATIONAL JOURNAL OF CANCER, 136(10), 2464-2468 [10.1002/ijc.29285].

HLA-DRB1∗13:01 allele in the genetic susceptibility to colorectal carcinoma

BUONOMO, ORESTE CLAUDIO;SILERI, PIERPAOLO;ARRIGA, ROBERTO;ORLANDI, AUGUSTO;LAURO, DAVIDE;ROSSI, PIERO;
2015-05-01

Abstract

Increasing evidence suggests that HLA-DRB1 alleles reduce or increase the risk of developing ulcerative colitis-associated colorectal carcinoma (CRC) tumors. However, the role of HLA-DRB1 locus on the susceptibility to develop CRC tumor, in the absence of a history of inflammatory bowel diseases (IBDs), is unclear. The aim of our study was to determine whether HLA-DRB1 alleles are associated with IBD-independent CRC tumor. HLA-DRB1 allele polymorphisms were identified by sequence-based typing method in 53 CRC patients and 57 sex- and age-matched healthy Caucasian controls. Pearson's chi-squared analysis with Yate's correction or Fisher's exact test with Bonferroni's correction, as appropriate, were used to compare the allele frequency (AF) differences of HLA-DRB1 in patients and controls. A total of 29 HLA-DRB1 alleles were recognized. A detailed study of these alleles allowed to identify DRB1*13:01 and DRB1*11:01 alleles that were significantly associated with an increased and reduced risk to develop CRC tumor, respectively. AF of DRB1*13:01, in CRC patients, was significantly higher than that of healthy controls, even following Bonferroni's correction (p = 0.029). In contrast, the presence of the DRB1*11:01 allele was negatively associated with CRC tumor as evidenced by the significantly lower AF in CRC patients than that of healthy controls (p = 0.005). However, following Bonferroni's correction, the AF of DRB*11:01 lost its statistical significance. These results suggest that HLA-DRB1*13:01 allele could be a potential marker for predicting genetic susceptibility to CRC tumor. In contrast, the protective role of DRB1*11:01 remains unclear.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/12 - Gastroenterologia
Settore MED/13 - Endocrinologia
English
Con Impact Factor ISI
DRB1; HLA; HLA-DRB1*11:01; HLA-DRB1*13:01; PCR-SBT; colorectal cancer; Adult; Aged; Aged, 80 and over; Case-Control Studies; Colorectal Neoplasms; European Continental Ancestry Group; Female; Gene Frequency; Genetic Variation; HLA-DRB1 Chains; Histocompatibility Testing; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide; Risk Factors; Sequence Analysis, DNA; Tumor Markers, Biological; Genetic Predisposition to Disease
Aureli, A., Canossi, A., Del Beato, T., Franceschilli, L., Buonomo, O.c., Papola, F., et al. (2015). HLA-DRB1∗13:01 allele in the genetic susceptibility to colorectal carcinoma. INTERNATIONAL JOURNAL OF CANCER, 136(10), 2464-2468 [10.1002/ijc.29285].
Aureli, A; Canossi, A; Del Beato, T; Franceschilli, L; Buonomo, Oc; Papola, F; De Sanctis, F; Lanzilli, G; Sileri, P; Coppola, A; Caratelli, S; Arriga, R; Orlandi, A; Lauro, D; Rossi, P; Sconocchia, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/120016
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