Double-stranded RNA-mediated interference (RNAi) is a new simple and fast research tool for shutting 7 down genes and characterizes function of their respective proteins. Many strategies for design and delivery of 8 siRNA to target cells are available. Here, we describe the use of lentiviral short hairpin RNA (shRNA) RNA 9 silencing to identify the involvement of d-serine racemase (SR)- an enzyme that syntheses d-serine to modu- 10 late glutamate-N-methyl-d-aspartate receptor- in regulating rat cerebellar granule neurons (CGN) apoptosis. 11 Apoptosis is induced by serum and KCl withdrawal and is detected with fluorometric caspase 3 assay.
Canu, N. (2015). Design and Cloning of Short HairpinRNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis. In Laura Lossi e Adalberto Merighi (a cura di), Neuronal Cell Death: Methods and Protocols, Methods in Molecular Biology. New YorK : Springer Science+Business Media New York 2015 [10.1007/978-1-4939-2152-2_9].
Design and Cloning of Short HairpinRNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis
CANU, NADIA
2015-01-01
Abstract
Double-stranded RNA-mediated interference (RNAi) is a new simple and fast research tool for shutting 7 down genes and characterizes function of their respective proteins. Many strategies for design and delivery of 8 siRNA to target cells are available. Here, we describe the use of lentiviral short hairpin RNA (shRNA) RNA 9 silencing to identify the involvement of d-serine racemase (SR)- an enzyme that syntheses d-serine to modu- 10 late glutamate-N-methyl-d-aspartate receptor- in regulating rat cerebellar granule neurons (CGN) apoptosis. 11 Apoptosis is induced by serum and KCl withdrawal and is detected with fluorometric caspase 3 assay.| File | Dimensione | Formato | |
|---|---|---|---|
|
302257_1_En_9 REVISED.pdf
accesso aperto
Descrizione: capitolo
Licenza:
Copyright dell'editore
Dimensione
440.43 kB
Formato
Adobe PDF
|
440.43 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
