Double-stranded RNA-mediated interference (RNAi) is a new simple and fast research tool for shutting 7 down genes and characterizes function of their respective proteins. Many strategies for design and delivery of 8 siRNA to target cells are available. Here, we describe the use of lentiviral short hairpin RNA (shRNA) RNA 9 silencing to identify the involvement of d-serine racemase (SR)- an enzyme that syntheses d-serine to modu- 10 late glutamate-N-methyl-d-aspartate receptor- in regulating rat cerebellar granule neurons (CGN) apoptosis. 11 Apoptosis is induced by serum and KCl withdrawal and is detected with fluorometric caspase 3 assay.

Canu, N. (2015). Design and Cloning of Short HairpinRNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis. In Laura Lossi e Adalberto Merighi (a cura di), Neuronal Cell Death: Methods and Protocols, Methods in Molecular Biology. New YorK : Springer Science+Business Media New York 2015 [10.1007/978-1-4939-2152-2_9].

Design and Cloning of Short HairpinRNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis

CANU, NADIA
2015

Abstract

Double-stranded RNA-mediated interference (RNAi) is a new simple and fast research tool for shutting 7 down genes and characterizes function of their respective proteins. Many strategies for design and delivery of 8 siRNA to target cells are available. Here, we describe the use of lentiviral short hairpin RNA (shRNA) RNA 9 silencing to identify the involvement of d-serine racemase (SR)- an enzyme that syntheses d-serine to modu- 10 late glutamate-N-methyl-d-aspartate receptor- in regulating rat cerebellar granule neurons (CGN) apoptosis. 11 Apoptosis is induced by serum and KCl withdrawal and is detected with fluorometric caspase 3 assay.
Settore BIO/09
English
Rilevanza internazionale
Capitolo o saggio
siRNA, ShRNA, Lentivirus, Cerebellar granule cells, d-serine racemase
http://www.springer.com/biomed/neuroscience/book/978-1-4939-2151-5
Canu, N. (2015). Design and Cloning of Short HairpinRNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis. In Laura Lossi e Adalberto Merighi (a cura di), Neuronal Cell Death: Methods and Protocols, Methods in Molecular Biology. New YorK : Springer Science+Business Media New York 2015 [10.1007/978-1-4939-2152-2_9].
Canu, N
Contributo in libro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/99310
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