Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.

Tong, A., Drees, B., Nardelli, G., Bader, G., Brannetti, B., Castagnoli, L., et al. (2002). A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules. SCIENCE, 295(5553), 321-324 [10.1126/science.1064987].

A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules

CASTAGNOLI, LUISA;PAOLUZI, SERENA;CESARENI, GIOVANNI
2002-01-11

Abstract

Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.
11-gen-2002
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/18 - GENETICA
English
Con Impact Factor ISI
Software; Peptide Library; Two-Hybrid System Techniques; Saccharomyces cerevisiae Proteins; Algorithms; Amino Acid Sequence; Wiskott-Aldrich Syndrome Protein; Computational Biology; Protein Binding; Fungal Proteins; src Homology Domains; Saccharomyces cerevisiae; Binding Sites; Amino Acid Motifs; Databases, Protein; Proteome; Molecular Sequence Data; Proteins; Cytoskeletal Proteins; Peptides; Consensus Sequence; Databases, Genetic; Protein Structure, Tertiary; Ligands
Tong, A., Drees, B., Nardelli, G., Bader, G., Brannetti, B., Castagnoli, L., et al. (2002). A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules. SCIENCE, 295(5553), 321-324 [10.1126/science.1064987].
Tong, A; Drees, B; Nardelli, G; Bader, G; Brannetti, B; Castagnoli, L; Evangelista, M; Ferracuti, S; Nelson, B; Paoluzi, S; Quondam, M; Zucconi, A; Hogue, C; Fields, S; Boone, C; Cesareni, G
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/96333
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 598
  • ???jsp.display-item.citation.isi??? 542
social impact