The reversible phosphorylation of tyrosine residues is one of the most frequent post-translational modifications regulating enzymatic activities and protein-protein interactions in eukaryotic cells. Cells responding to internal or external regulatory inputs modify their phosphorylation status and diseased cells can often be diagnosed by observing alterations in their qualitative or quantitative phosphorylation profile. As a consequence the ability to describe the phosphorylation profile of a cell is central to many approaches aiming at the characterisation of signalling pathways. Anti-phosphotyrosine (pY) antibodies are widely used as experimental tools to monitor the phosphorylation status of a cell. By using peptide microarray technology we have characterised the substrate specificity of three widely used pY antibodies. We report that they are more sensitive to sequence context than is generally assumed and that their sequence preferences differ.

Tinti, M., Castagnoli, L., Nardozza, A., Ferrari, E., Cesareni, G., Sacco, F., et al. (2012). The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays. NEW BIOTECHNOLOGY, 29(5), 571-577 [10.1016/j.nbt.2011.12.001].

The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays

TINTI, MICHELE;CASTAGNOLI, LUISA;CESARENI, GIOVANNI;Sacco, F;
2012-06-15

Abstract

The reversible phosphorylation of tyrosine residues is one of the most frequent post-translational modifications regulating enzymatic activities and protein-protein interactions in eukaryotic cells. Cells responding to internal or external regulatory inputs modify their phosphorylation status and diseased cells can often be diagnosed by observing alterations in their qualitative or quantitative phosphorylation profile. As a consequence the ability to describe the phosphorylation profile of a cell is central to many approaches aiming at the characterisation of signalling pathways. Anti-phosphotyrosine (pY) antibodies are widely used as experimental tools to monitor the phosphorylation status of a cell. By using peptide microarray technology we have characterised the substrate specificity of three widely used pY antibodies. We report that they are more sensitive to sequence context than is generally assumed and that their sequence preferences differ.
15-giu-2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/18 - GENETICA
English
Protein Array Analysis; Antibodies, Phospho-Specific; Antibody Specificity; Signal Processing, Computer-Assisted; Reproducibility of Results; Phosphorylation; Humans; HEK293 Cells; Proteomics; Molecular Sequence Data; Amino Acid Sequence; Phosphopeptides
Tinti, M., Castagnoli, L., Nardozza, A., Ferrari, E., Cesareni, G., Sacco, F., et al. (2012). The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays. NEW BIOTECHNOLOGY, 29(5), 571-577 [10.1016/j.nbt.2011.12.001].
Tinti, M; Castagnoli, L; Nardozza, A; Ferrari, E; Cesareni, G; Sacco, F; Corallino, S; Castagnoli, L; Cesareni, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/96313
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