4-Hydroxynonenal (HNE) is a highly reactive aldehyde, produced by cellular lipid peroxidation, able to inhibit proliferation and to induce differentiation in MEL cells at concentrations similar to those detected in several normal tissues. Inducer-mediated differentiation of murine erythroleukemia (MEL) cells is a multiple step process characterized by modulation of several genes as well as by a transient increase in the amount of membrane-associated protein kinase C (PKC) activity. Here we demonstrate that a rapid translocation of PKC activity from cytosol to the membranes occurs during the differentiation induced by HNE. When PKC is completely translocated by phorbol-12-myristate-13-acetate (TPA), the degree of HNE-induced MEL cells differentiation is highly decreased. However, if TPA is washed out from the culture medium before the exposition to the aldehyde, HNE gradually resumes its differentiative ability. The incubation of cells with a selective inhibitor of PKC activity, bisindolylmaleimide GF 109203X, partially prevents the HNE-induced differentiation in MEL cells. In conclusion, our results demonstrate that HNE-induced MEL cell differentiation is preceded by a rapid translocation of PKC activity, and that the inhibition of this phenomenon prevents the onset of terminal differentiation.
Rinaldi, M., Barrera, G., Aquino, A., Spinsanti, P., Pizzimenti, S., Farace, M.G., et al. (2000). 4-Hydroxynonenal-induced MEL cell differentiation involves PKC activity translocation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 272(1), 75-80.
|Tipologia:||Articolo su rivista|
|Citazione:||Rinaldi, M., Barrera, G., Aquino, A., Spinsanti, P., Pizzimenti, S., Farace, M.G., et al. (2000). 4-Hydroxynonenal-induced MEL cell differentiation involves PKC activity translocation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 272(1), 75-80.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/bbrc.2000.2691|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||27-mag-2000|
|Titolo:||4-Hydroxynonenal-induced MEL cell differentiation involves PKC activity translocation|
|Autori:||Rinaldi, M; Barrera, G; Aquino, A; Spinsanti, P; Pizzimenti, S; Farace, MG; Dianzani, MU; Fazio, VM|
|Appare nelle tipologie:||01 - Articolo su rivista|