Adenosine deaminases that act on dsRNA (ADARs) are enzymes that target double-stranded regions of RNA converting adenosines into inosines (A-to-I editing) thus contributing to genome complexity and fine regulation of gene expression. It has been described that a member of the ADAR family, ADAR1, can target viruses and affect their replication process. Here we report evidence showing that ADAR1 stimulates human immuno deficiency virus type 1 (HIV-1) replication by using both editing-dependent and editing-independent mechanisms. We show that over-expression of ADAR1 in HIV-1 producer cells increases viral protein accumulation in an editing-independent manner. Moreover, HIV-1 virions generated in the presence of over-expressed ADAR1 but not an editing-inactive ADAR1 mutant are released more efficiently and display enhanced infectivity, as demonstrated by challenge assays performed with T cell lines and primary CD4(+) T lymphocytes. Finally, we report that ADAR1 associates with HIV-1 RNAs and edits adenosines in the 5' untranslated region (UTR) and the Rev and Tat coding sequence. Overall these results suggest that HIV-1 has evolved mechanisms to take advantage of specific RNA editing activity of the host cell and disclose a stimulatory function of ADAR1 in the spread of HIV-1.
Doria, M., Neri, F., Gallo, A., Farace, M.G., & Michienzi, A. (2009). Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection. NUCLEIC ACIDS RESEARCH, 37(17), 5848-5858.
|Tipologia:||Articolo su rivista|
|Citazione:||Doria, M., Neri, F., Gallo, A., Farace, M.G., & Michienzi, A. (2009). Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection. NUCLEIC ACIDS RESEARCH, 37(17), 5848-5858.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/nar/gkp604|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||set-2009|
|Titolo:||Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection|
|Autori:||Doria, M; Neri, F; Gallo, A; Farace, MG; Michienzi, A|
|Appare nelle tipologie:||01 - Articolo su rivista|