Platelets are stored at 22 degrees C, since incubation at 37 degrees C results in loss of viability. Nonetheless, in our body (37 degrees C), platelets survive for 8-10 days. This discrepancy has been explained in terms of deprivation of viability factors or accumulation of apoptotic factors during storage. We report that the endocannabinoid anandamide (AEA) may be one of the agents allowing platelet survival. In fact, at 37 degrees C, human platelets enhance the expression of pro-apoptotic proteins (caspases, Bax, Bak) and decrease the expression of Bcl-xL, thus changing the Bcl-xL/Bak ratio, a key platelet biological clock. AEA or its non-hydrolyzable analogue, methanandamide, extend platelet life span, without reversing the changes in Bcl-xL/Bak ratio induced by heat stress. Instead, AEA binding to type-1 cannabinoid receptor activates Akt, which regulates, through phosphorylation of Bad, the interactions among different Bcl-2 family members. These findings could have implications for platelet collection and, potentially, for their clinical use.

Catani, M.v., Gasperi, V., Evangelista, D., FINAZZI AGRO', A., Avigliano, L., Maccarrone, M. (2010). Anandamide extends platelets survival through CB(1)-dependent Akt signaling. CELLULAR AND MOLECULAR LIFE SCIENCES, 67(4), 601-610 [10.1007/s00018-009-0198-9].

Anandamide extends platelets survival through CB(1)-dependent Akt signaling

CATANI, MARIA VALERIA;GASPERI, VALERIA;FINAZZI AGRO', ALESSANDRO;AVIGLIANO, LUCIANA;
2010-02-01

Abstract

Platelets are stored at 22 degrees C, since incubation at 37 degrees C results in loss of viability. Nonetheless, in our body (37 degrees C), platelets survive for 8-10 days. This discrepancy has been explained in terms of deprivation of viability factors or accumulation of apoptotic factors during storage. We report that the endocannabinoid anandamide (AEA) may be one of the agents allowing platelet survival. In fact, at 37 degrees C, human platelets enhance the expression of pro-apoptotic proteins (caspases, Bax, Bak) and decrease the expression of Bcl-xL, thus changing the Bcl-xL/Bak ratio, a key platelet biological clock. AEA or its non-hydrolyzable analogue, methanandamide, extend platelet life span, without reversing the changes in Bcl-xL/Bak ratio induced by heat stress. Instead, AEA binding to type-1 cannabinoid receptor activates Akt, which regulates, through phosphorylation of Bad, the interactions among different Bcl-2 family members. These findings could have implications for platelet collection and, potentially, for their clinical use.
feb-2010
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Catani, M.v., Gasperi, V., Evangelista, D., FINAZZI AGRO', A., Avigliano, L., Maccarrone, M. (2010). Anandamide extends platelets survival through CB(1)-dependent Akt signaling. CELLULAR AND MOLECULAR LIFE SCIENCES, 67(4), 601-610 [10.1007/s00018-009-0198-9].
Catani, Mv; Gasperi, V; Evangelista, D; FINAZZI AGRO', A; Avigliano, L; Maccarrone, M
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/9291
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 28
  • ???jsp.display-item.citation.isi??? 25
social impact