4-Hydroxynonenal (HNE) is one of the major end products of lipid peroxidation. Here we show that the exposure of murine erythroleukemia (MEL) cells to 1 microM HNE, for 10.5 h over 2 days, induces a differentiation comparable with that observed in cells exposed to DMSO for the whole experiment (7 days). The exposure of MEL cells for the same length of time demonstrates a higher degree of differentiation in HNE-treated than in DMSO-treated MEL cells. The protooncogene c-myc is down-modulated early, in HNE-induced MEL cells as well as in DMSO-treated cells. However, ornithine decarboxylase gene expression first increases and then decreases, during the lowering of the proliferation rate. These findings indicate that HNE, at a concentration physiologically found in many normal tissues and in the plasma, induces MEL cell differentiation by modulation of specific gene expression.

Rinaldi, M., Barrera, G., Spinsanti, P., Pizzimenti, S., Ciafre', S.a., Parrella, P., et al. (2001). Growth inhibition and differentiation induction in murine erythroleukemia cells by 4-hydroxynonenal. FREE RADICAL RESEARCH, 34(6), 629-637.

Growth inhibition and differentiation induction in murine erythroleukemia cells by 4-hydroxynonenal

CIAFRE', SILVIA ANNA;FARACE, MARIA GIULIA;
2001-06-01

Abstract

4-Hydroxynonenal (HNE) is one of the major end products of lipid peroxidation. Here we show that the exposure of murine erythroleukemia (MEL) cells to 1 microM HNE, for 10.5 h over 2 days, induces a differentiation comparable with that observed in cells exposed to DMSO for the whole experiment (7 days). The exposure of MEL cells for the same length of time demonstrates a higher degree of differentiation in HNE-treated than in DMSO-treated MEL cells. The protooncogene c-myc is down-modulated early, in HNE-induced MEL cells as well as in DMSO-treated cells. However, ornithine decarboxylase gene expression first increases and then decreases, during the lowering of the proliferation rate. These findings indicate that HNE, at a concentration physiologically found in many normal tissues and in the plasma, induces MEL cell differentiation by modulation of specific gene expression.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
Leukemia, Erythroblastic, Acute; Gene Expression; Growth Inhibitors; Tumor Cells, Cultured; Aldehydes; Mice; Cell Differentiation; Ornithine Decarboxylase; Genes, myc; Cell Division; Animals
Rinaldi, M., Barrera, G., Spinsanti, P., Pizzimenti, S., Ciafre', S.a., Parrella, P., et al. (2001). Growth inhibition and differentiation induction in murine erythroleukemia cells by 4-hydroxynonenal. FREE RADICAL RESEARCH, 34(6), 629-637.
Rinaldi, M; Barrera, G; Spinsanti, P; Pizzimenti, S; Ciafre', Sa; Parrella, P; Farace, Mg; Signori, E; Dianzani, M; Fazio, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/9198
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