Reelin is an extracellular matrix protein that plays a pivotal role in development of the central nervous system. Reelin is also expressed in the adult brain, notably in the cerebral cortex, where it might play a role in synaptic plasticity. The mechanism of action of reelin at the molecular level has been the subject of several hypotheses. Here we show that reelin is a serine protease and that proteolytic activity is relevant to its function, since (i) Reelin expression in HEK 293T cells impairs their ability to adhere to fibronectin-coated surfaces, and adhesion to fibronectin is restored by micromolar concentrations of diisopropyl phosphorofluoridate, a serine hydrolase inhibitor; (ii) purified Reelin binds FP-Peg-biotin, a trap probe which irreversibly binds to serine residues located in active catalytic sites of serine hydrolases; (iii) purified Reelin rapidly degrades fibronectin and laminin, while collagen IV is degraded at a much slower rate; fibronectin degradation is inhibited by inhibitors of serine proteases, and by monoclonal antibody CR-50, an antibody known to block the function of Reelin both in vitro and in vivo. The proteolytic activity of Reelin on adhesion molecules of the extracellular matrix and/or receptors on neurons may explain how Reelin regulates neuronal migration and synaptic plasticity.
Quattrocchi, C.C., Wannenes, F., Persico, A.M., Ciafré, S.A., D'Arcangelo, G., Farace, M.G., et al. (2002). Reelin is a serine protease of the extracellular matrix. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 277(1), 303-309 [10.1074/jbc.M106996200].
|Tipologia:||Articolo su rivista|
|Citazione:||Quattrocchi, C.C., Wannenes, F., Persico, A.M., Ciafré, S.A., D'Arcangelo, G., Farace, M.G., et al. (2002). Reelin is a serine protease of the extracellular matrix. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 277(1), 303-309 [10.1074/jbc.M106996200].|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1074/jbc.M106996200|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||4-gen-2002|
|Titolo:||Reelin is a serine protease of the extracellular matrix|
|Autori:||Quattrocchi, CC; Wannenes, F; Persico, AM; Ciafré, SA; D'Arcangelo, G; Farace, MG; Keller, F|
|Appare nelle tipologie:||01 - Articolo su rivista|