We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.
Svicher, V., Alteri, C., Montano, M., Nori, A., D'Arrigo, R., Andreoni, M., et al. (2014). Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group. INFECTION, 42(1), 61-71 [10.1007/s15010-013-0510-3].
Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group
SVICHER, VALENTINA;MONTANO, MARCO;ANDREONI, MASSIMO;BERTOLI, ADA;CECCHERINI SILBERSTEIN, FRANCESCA;DI SANTO, FABIOLA;MAFFONGELLI, GAETANO;VULLO, VINCENZO;PERNO, CARLO FEDERICO
2014-02-01
Abstract
We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.| File | Dimensione | Formato | |
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