In this work a novel bioassay for mustard agent detection was proposed. The bioassay is based on the capability of these compounds to inhibit the enzyme choline oxidase. The enzymatic activity, which is correlated to the mustard agents, was electrochemically monitored measuring the enzymatic product, hydrogen peroxide, by means of a screen-printed electrode modified with Prussian Blue nanoparticles. Prussian Blue nanoparticles are able to electrocatalyse the hydrogen peroxide concentration reduction at low applied potential (−50 mV vs. Ag/AgCl), thus allowing the detection of the mustard agents with no electrochemical interferences. The suitability of this novel bioassay was tested with the nitrogen mustard simulant bis(2-chloroethyl)amine and the sulfur mustard simulants 2-chloroethyl ethyl sulfide and 2-chloroethyl phenyl sulfide. The bioassay proposed in this work allowed the detection of mustard agent simulants with good sensitivity and fast response, which are excellent premises for the development of a miniaturised sensor well suited for an alarm system in case of terrorist attacks.

Arduini, F., Scognamiglio, V., Covaia, C., Amine, A., MOSCONE DINIA, D., Palleschi, G. (2015). A Choline oxidase amperometric bioassay for the detection of mustard agents based on screen-printed electrodes modified with Prussian blue nanoparticles. SENSORS, 15(2), 4353-4367 [10.3390/s150204353].

A Choline oxidase amperometric bioassay for the detection of mustard agents based on screen-printed electrodes modified with Prussian blue nanoparticles

ARDUINI, FABIANA;MOSCONE DINIA, DANILA;PALLESCHI, GIUSEPPE
2015-01-01

Abstract

In this work a novel bioassay for mustard agent detection was proposed. The bioassay is based on the capability of these compounds to inhibit the enzyme choline oxidase. The enzymatic activity, which is correlated to the mustard agents, was electrochemically monitored measuring the enzymatic product, hydrogen peroxide, by means of a screen-printed electrode modified with Prussian Blue nanoparticles. Prussian Blue nanoparticles are able to electrocatalyse the hydrogen peroxide concentration reduction at low applied potential (−50 mV vs. Ag/AgCl), thus allowing the detection of the mustard agents with no electrochemical interferences. The suitability of this novel bioassay was tested with the nitrogen mustard simulant bis(2-chloroethyl)amine and the sulfur mustard simulants 2-chloroethyl ethyl sulfide and 2-chloroethyl phenyl sulfide. The bioassay proposed in this work allowed the detection of mustard agent simulants with good sensitivity and fast response, which are excellent premises for the development of a miniaturised sensor well suited for an alarm system in case of terrorist attacks.
2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
mustard agents; inhibition; choline oxidase; screen-printed electrode; Prussian Blue nanoparticles
Arduini, F., Scognamiglio, V., Covaia, C., Amine, A., MOSCONE DINIA, D., Palleschi, G. (2015). A Choline oxidase amperometric bioassay for the detection of mustard agents based on screen-printed electrodes modified with Prussian blue nanoparticles. SENSORS, 15(2), 4353-4367 [10.3390/s150204353].
Arduini, F; Scognamiglio, V; Covaia, C; Amine, A; MOSCONE DINIA, D; Palleschi, G
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/89227
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