Acanthamoeba keratitis (AK) is an ocular disease caused by members of a genus of free-living amoebae and it is associated predominantly with contact lens (CL) use. This study reports 55 cases of AK diagnosed in Italy. Genotype identification was carried out by PCR assay followed by sequence analysis of the 18S rRNA gene using the genus specific primers JDP1 and JDP2. Genotype assignment was based on phenetic analysis of the ASA.S1 subset of the small-subunit rRNA gene sequences. The material has been collected at the Polyclinic Tor Vergata of Rome for a total of 19 isolates and at the Polyclinic Hospital of Bari (36 isolates). Thirty-three out of the 55 genetically characterized isolates were assigned to the genotype T4. Ten isolates were identified as belonging to the genotype T15 thus confirming the first association between the genotype T15 and human amoebic keratitis previously described from the same area. We underline the occurrence of the genotype T3 and T11 identified for the first time in the country.

DI CAVE, D., D'Alfonso, R., Dussey Comlavi, K., D' Orazi, C., Monno, R., Berrilli, F. (2014). Genotypic heterogeneity based on 18S-rRNA gene sequences among Acanthamoeba isolates from clinical samples in Italy. EXPERIMENTAL PARASITOLOGY, 145 suppl, 46-49 [10.1016/j.exppara.2014.05.009].

Genotypic heterogeneity based on 18S-rRNA gene sequences among Acanthamoeba isolates from clinical samples in Italy

DI CAVE, DAVID;D'ALFONSO, ROSSELLA;BERRILLI, FEDERICA
2014-05-20

Abstract

Acanthamoeba keratitis (AK) is an ocular disease caused by members of a genus of free-living amoebae and it is associated predominantly with contact lens (CL) use. This study reports 55 cases of AK diagnosed in Italy. Genotype identification was carried out by PCR assay followed by sequence analysis of the 18S rRNA gene using the genus specific primers JDP1 and JDP2. Genotype assignment was based on phenetic analysis of the ASA.S1 subset of the small-subunit rRNA gene sequences. The material has been collected at the Polyclinic Tor Vergata of Rome for a total of 19 isolates and at the Polyclinic Hospital of Bari (36 isolates). Thirty-three out of the 55 genetically characterized isolates were assigned to the genotype T4. Ten isolates were identified as belonging to the genotype T15 thus confirming the first association between the genotype T15 and human amoebic keratitis previously described from the same area. We underline the occurrence of the genotype T3 and T11 identified for the first time in the country.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
Settore BIO/12
English
Con Impact Factor ISI
Acanthamoeba; Keratitis; 18S rDNA; Italy; Genotype variability
DI CAVE, D., D'Alfonso, R., Dussey Comlavi, K., D' Orazi, C., Monno, R., Berrilli, F. (2014). Genotypic heterogeneity based on 18S-rRNA gene sequences among Acanthamoeba isolates from clinical samples in Italy. EXPERIMENTAL PARASITOLOGY, 145 suppl, 46-49 [10.1016/j.exppara.2014.05.009].
DI CAVE, D; D'Alfonso, R; Dussey Comlavi, K; D' Orazi, C; Monno, R; Berrilli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/88852
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