The effects of glycine on non-dopaminergic cells in rat substantia nigra pars compacta and pars reticulata maintained in vitro were investigated using intracellular recording techniques. Glycine, superfused at a concentration between 30 microM and 1 mM, reversibly blocked the spontaneous firing of these neurons. The inhibition of firing discharge was associated with a hyperpolarization of the membrane (potassium acetate-filled electrodes) and an increase in conductance. Under voltage-clamp experiments (holding potential between -57 and -65 mV), glycine produced an outward response which reversed polarity at about -74 mV. However, when the recording electrodes were filled with KCl, the glycinergic response was mainly depolarizing/inward and reversed at about -43 mV. Thus, it appeared to be due to an increase in chloride permeability. Furthermore, the effects of glycine were reversibly antagonized by strychnine (between 300 nM and 1 microM). Our findings demonstrate that glycine is a potent inhibitory agent on non-dopaminergic cells of the substantia pars compacta and par reticulata that acts by activating strychnine-sensitive receptors.
Mercuri, N., Bonci, A., Pisani, A., Calabresi, P., & Bernardi, G. (1995). Actions of glycine on non-dopaminergic neurons of the rat substantia nigra. EUROPEAN JOURNAL OF NEUROSCIENCE, 7(11), 2351-2354.
|Tipologia:||Articolo su rivista|
|Citazione:||Mercuri, N., Bonci, A., Pisani, A., Calabresi, P., & Bernardi, G. (1995). Actions of glycine on non-dopaminergic neurons of the rat substantia nigra. EUROPEAN JOURNAL OF NEUROSCIENCE, 7(11), 2351-2354.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Revisione (peer review):||Esperti anonimi|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1-nov-1995|
|Titolo:||Actions of glycine on non-dopaminergic neurons of the rat substantia nigra|
|Autori:||Mercuri, N; Bonci, A; Pisani, A; Calabresi, P; Bernardi, G|
|Appare nelle tipologie:||01 - Articolo su rivista|