The effects of glycine on non-dopaminergic cells in rat substantia nigra pars compacta and pars reticulata maintained in vitro were investigated using intracellular recording techniques. Glycine, superfused at a concentration between 30 microM and 1 mM, reversibly blocked the spontaneous firing of these neurons. The inhibition of firing discharge was associated with a hyperpolarization of the membrane (potassium acetate-filled electrodes) and an increase in conductance. Under voltage-clamp experiments (holding potential between -57 and -65 mV), glycine produced an outward response which reversed polarity at about -74 mV. However, when the recording electrodes were filled with KCl, the glycinergic response was mainly depolarizing/inward and reversed at about -43 mV. Thus, it appeared to be due to an increase in chloride permeability. Furthermore, the effects of glycine were reversibly antagonized by strychnine (between 300 nM and 1 microM). Our findings demonstrate that glycine is a potent inhibitory agent on non-dopaminergic cells of the substantia pars compacta and par reticulata that acts by activating strychnine-sensitive receptors.

Mercuri, N., Bonci, A., Pisani, A., Calabresi, P., & Bernardi, G. (1995). Actions of glycine on non-dopaminergic neurons of the rat substantia nigra. EUROPEAN JOURNAL OF NEUROSCIENCE, 7(11), 2351-2354.

Actions of glycine on non-dopaminergic neurons of the rat substantia nigra

MERCURI, NICOLA BIAGIO;PISANI, ANTONIO;CALABRESI, PAOLO;BERNARDI, GIORGIO
1995-11-01

Abstract

The effects of glycine on non-dopaminergic cells in rat substantia nigra pars compacta and pars reticulata maintained in vitro were investigated using intracellular recording techniques. Glycine, superfused at a concentration between 30 microM and 1 mM, reversibly blocked the spontaneous firing of these neurons. The inhibition of firing discharge was associated with a hyperpolarization of the membrane (potassium acetate-filled electrodes) and an increase in conductance. Under voltage-clamp experiments (holding potential between -57 and -65 mV), glycine produced an outward response which reversed polarity at about -74 mV. However, when the recording electrodes were filled with KCl, the glycinergic response was mainly depolarizing/inward and reversed at about -43 mV. Thus, it appeared to be due to an increase in chloride permeability. Furthermore, the effects of glycine were reversibly antagonized by strychnine (between 300 nM and 1 microM). Our findings demonstrate that glycine is a potent inhibitory agent on non-dopaminergic cells of the substantia pars compacta and par reticulata that acts by activating strychnine-sensitive receptors.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/26 - Neurologia
English
Con Impact Factor ISI
Rats; Animals; Dopamine; Glycine; Dose-Response Relationship, Drug; Neurons; Rats, Wistar; Membrane Potentials; Substantia Nigra
Mercuri, N., Bonci, A., Pisani, A., Calabresi, P., & Bernardi, G. (1995). Actions of glycine on non-dopaminergic neurons of the rat substantia nigra. EUROPEAN JOURNAL OF NEUROSCIENCE, 7(11), 2351-2354.
Mercuri, Nb; Bonci, A; Pisani, A; Calabresi, P; Bernardi, G
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/88318
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact