Intracellular pH and membrane potential were measured during hypoxia and/or hypoglycaemia in cortical pyramidal neurones of a rat cortical slice preparation. Intracellular pH (pHi) was calculated from ratiometric microfluorometry of the pH-sensitive dye BCECF injected via sharp recording microelectrodes into the neurones. Transient (5 min) hypoxia induced a fall of pHi (7.12 +/- 0.03) of -0.72 +/- 0.11 pH units while transient (10 min) hypoglycaemia induced an increase of 0.37 +/- 0.09 pH units. Hypoglycaemia did not prevent the hypoxic acidification. Lowering extracellular Na+ induced a membrane hyperpolarization and alkalinization by 0.29 +/- 0.12 pH units but did not affect the development or recovery of the hypoxic acidification. The alkalinization during hypoglycaemia suggested that there is some anaerobic glycolysis under normoglycemic conditions. The hypoxic acidification, however, is unlikely to result from anaerobic glycolysis or reversal of Na(+)-dependent H+ extrusion.

Knöpfel, T., Tozzi, A., Pisani, A., Calabresi, P., Bernardi, G. (1998). Hypoxic and hypoglycaemic changes of intracellular pH in cerebral cortical pyramidal neurones. NEUROREPORT, 9(7), 1447-1450.

Hypoxic and hypoglycaemic changes of intracellular pH in cerebral cortical pyramidal neurones

PISANI, ANTONIO;CALABRESI, PAOLO;BERNARDI, GIORGIO
1998-05-11

Abstract

Intracellular pH and membrane potential were measured during hypoxia and/or hypoglycaemia in cortical pyramidal neurones of a rat cortical slice preparation. Intracellular pH (pHi) was calculated from ratiometric microfluorometry of the pH-sensitive dye BCECF injected via sharp recording microelectrodes into the neurones. Transient (5 min) hypoxia induced a fall of pHi (7.12 +/- 0.03) of -0.72 +/- 0.11 pH units while transient (10 min) hypoglycaemia induced an increase of 0.37 +/- 0.09 pH units. Hypoglycaemia did not prevent the hypoxic acidification. Lowering extracellular Na+ induced a membrane hyperpolarization and alkalinization by 0.29 +/- 0.12 pH units but did not affect the development or recovery of the hypoxic acidification. The alkalinization during hypoglycaemia suggested that there is some anaerobic glycolysis under normoglycemic conditions. The hypoxic acidification, however, is unlikely to result from anaerobic glycolysis or reversal of Na(+)-dependent H+ extrusion.
11-mag-1998
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/26 - NEUROLOGIA
English
Con Impact Factor ISI
Animals; Pyramidal Cells; Hydrogen-Ion Concentration; Fluoresceins; Fluorescent Dyes; Cell Hypoxia; Hypoglycemia; Cerebral Cortex; Rats; Rats, Wistar; Membrane Potentials; Time Factors; Male; Microelectrodes
Knöpfel, T., Tozzi, A., Pisani, A., Calabresi, P., Bernardi, G. (1998). Hypoxic and hypoglycaemic changes of intracellular pH in cerebral cortical pyramidal neurones. NEUROREPORT, 9(7), 1447-1450.
Knöpfel, T; Tozzi, A; Pisani, A; Calabresi, P; Bernardi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/88311
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