Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-γ measured by QFT-IT and the frequency of CD4+/CD69+/IFN-γ+ T cells. The frequency of CD4+/CD69+/IFNγ+ responding T cells was higher in active-TB patients (0.254 ± 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFNγ+ T cells was significantly higher in RC-TB (0.245 ± 0.305%, P < 0.05) compared to the other study groups.

Nikolova, M., Markova, R., Drenska, R., Muhtarova, M., Todorova, Y., Dimitrov, V., et al. (2013). Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 75(3), 277-281 [10.1016/j.diagmicrobio.2012.11.023].

Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection

SALTINI, CESARE;AMICOSANTE, MASSIMO
2013-03-01

Abstract

Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-γ measured by QFT-IT and the frequency of CD4+/CD69+/IFN-γ+ T cells. The frequency of CD4+/CD69+/IFNγ+ responding T cells was higher in active-TB patients (0.254 ± 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFNγ+ T cells was significantly higher in RC-TB (0.245 ± 0.305%, P < 0.05) compared to the other study groups.
mar-2013
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - PATOLOGIA GENERALE
Settore MED/10 - MALATTIE DELL'APPARATO RESPIRATORIO
English
Con Impact Factor ISI
BCG Vaccine; Young Adult; Antigens, Differentiation, T-Lymphocyte; Interferon-gamma; Humans; Aged; CD4 Lymphocyte Count; CD4-Positive T-Lymphocytes; Cross-Sectional Studies; Lectins, C-Type; Aged, 80 and over; Antigens, CD; CD8-Positive T-Lymphocytes; Adult; Health Personnel; Case-Control Studies; Tuberculosis; Middle Aged; Flow Cytometry; Male; Biological Markers; Female; Mycobacterium tuberculosis
Nikolova, M., Markova, R., Drenska, R., Muhtarova, M., Todorova, Y., Dimitrov, V., et al. (2013). Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 75(3), 277-281 [10.1016/j.diagmicrobio.2012.11.023].
Nikolova, M; Markova, R; Drenska, R; Muhtarova, M; Todorova, Y; Dimitrov, V; Taskov, H; Saltini, C; Amicosante, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/88181
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