Bacterial endotoxin (lipopolysaccharide [LPS]) is a potent inducer of human dendritic cell (DC) maturation and survival. Here we show that immature DCs exposed to LPS trigger an early and sustained caspase-like activity, which can be blocked by zVAD (z-Val-Ala-Asp), in the absence of detectable caspase 8 and caspase 10 activation, or poly(ADP-ribose) polymerase (PARP)-cleaving activity. Preventing LPS-induced caspase-like activation in DC results in massive cell death. Importantly, triggering of the caspase-like activity is required for LPS-induced activation of extracellular signal-regulated kinases (ERKs) and for LPS-induced up-regulation of cFLIP (Fas-associating protein with death domain-like interleukin-1 beta-converting enzyme [FLICE]-like inhibitory protein). Therefore, a caspase-dependent pathway initiated by LPS controls survival of human DCs.

Franchi, L., Condo', I., Tomassini, B., Nicolò, C., Testi, R. (2003). A caspaselike activity is triggered by LPS and is required for survival of human dendritic cells. BLOOD, 102(8), 2910-5 [10.1182/blood-2003-03-0967].

A caspaselike activity is triggered by LPS and is required for survival of human dendritic cells

CONDO', IVANO;TESTI, ROBERTO
2003-10-15

Abstract

Bacterial endotoxin (lipopolysaccharide [LPS]) is a potent inducer of human dendritic cell (DC) maturation and survival. Here we show that immature DCs exposed to LPS trigger an early and sustained caspase-like activity, which can be blocked by zVAD (z-Val-Ala-Asp), in the absence of detectable caspase 8 and caspase 10 activation, or poly(ADP-ribose) polymerase (PARP)-cleaving activity. Preventing LPS-induced caspase-like activation in DC results in massive cell death. Importantly, triggering of the caspase-like activity is required for LPS-induced activation of extracellular signal-regulated kinases (ERKs) and for LPS-induced up-regulation of cFLIP (Fas-associating protein with death domain-like interleukin-1 beta-converting enzyme [FLICE]-like inhibitory protein). Therefore, a caspase-dependent pathway initiated by LPS controls survival of human DCs.
15-ott-2003
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - PATOLOGIA GENERALE
English
Con Impact Factor ISI
Protein Biosynthesis; Caspases; Time Factors; Peptides; Escherichia coli; Caspase 9; Dose-Response Relationship, Drug; Lipopolysaccharides; Carrier Proteins; Blotting, Western; Cell Separation; CASP8 and FADD-Like Apoptosis Regulating Protein; Mitogen-Activated Protein Kinases; Humans; Flow Cytometry; Dendritic Cells; Caspase 8; Enzyme Activation; Cell Survival; Intracellular Signaling Peptides and Proteins; Poly(ADP-ribose) Polymerases; Caspase 10
Franchi, L., Condo', I., Tomassini, B., Nicolò, C., Testi, R. (2003). A caspaselike activity is triggered by LPS and is required for survival of human dendritic cells. BLOOD, 102(8), 2910-5 [10.1182/blood-2003-03-0967].
Franchi, L; Condo', I; Tomassini, B; Nicolò, C; Testi, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/8808
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