In three-dimensional and four-dimensional experiments on doubly labelled proteins not only heteronuclear (13C or 15N) but also proton (1H) frequencies are often indirectly monitored, rather than being directly observed. In this communication we show how in these experiments by overlaying 1H and heteronuclear evolutions one can obtain decreased apparent relaxation rates of 1H signals, yielding improved sensitivity. The new method applies to spin pairs like 1H-15N, as in amide groups, or 1H-13C, as in methine groups of alpha or aromatic systems.
Bazzo, R., Barbato, G., Cicero, D.o. (2001). Improved Sensitivity in Indirect Monitoring of Chemical Shifts of Proton-Heteronuclear spin pairs (1H-13C and 1H-15N) in 3D and 4D NMR Spectroscopy. JOURNAL OF BIOMOLECULAR NMR, 19, 261-266.
Improved Sensitivity in Indirect Monitoring of Chemical Shifts of Proton-Heteronuclear spin pairs (1H-13C and 1H-15N) in 3D and 4D NMR Spectroscopy
CICERO, DANIEL OSCAR
2001-01-01
Abstract
In three-dimensional and four-dimensional experiments on doubly labelled proteins not only heteronuclear (13C or 15N) but also proton (1H) frequencies are often indirectly monitored, rather than being directly observed. In this communication we show how in these experiments by overlaying 1H and heteronuclear evolutions one can obtain decreased apparent relaxation rates of 1H signals, yielding improved sensitivity. The new method applies to spin pairs like 1H-15N, as in amide groups, or 1H-13C, as in methine groups of alpha or aromatic systems.File | Dimensione | Formato | |
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