This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.

Pietropolli, A., Vicario, R., Peconi, C., Zampatti, S., Quitadamo, M., Capogna, M., et al. (2014). Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis. THE JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 27(16), 1656-1660 [10.3109/14767058.2013.871697].

Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis

PIETROPOLLI, ADALGISA;ZAMPATTI, STEFANIA;SPITALIERI, PAOLA;NOVELLI, GIUSEPPE;SANGIUOLO, FEDERICA CARLA;PICCIONE, EMILIO;GIARDINA, EMILIANO
2014-04-09

Abstract

This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.
9-apr-2014
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/03 - GENETICA MEDICA
English
Pietropolli, A., Vicario, R., Peconi, C., Zampatti, S., Quitadamo, M., Capogna, M., et al. (2014). Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis. THE JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 27(16), 1656-1660 [10.3109/14767058.2013.871697].
Pietropolli, A; Vicario, R; Peconi, C; Zampatti, S; Quitadamo, M; Capogna, M; Ragazzo, M; Nardone, A; Postorivo, D; Spitalieri, P; Sarta, S; Ratto, F;...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/85894
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