Transcription factor expression levels, which sensitively reflect cellular development and disease state, are typically monitored via cumbersome, reagent-intensive assays that require relatively large quantities of cells. Here, we demonstrate a simple, quantitative approach to their detection based on a simple, electrochemical sensing platform. This sensor sensitively and quantitatively detects its target transcription factor in complex media (e.g., 250 μg/mL crude nuclear extracts) in a convenient, low-reagent process requiring only 10 μL of sample. Our approach thus appears a promising means of monitoring transcription factor levels.

Bonham, A., Hsieh, K., Ferguson, B., Vallée Bélisle, A., Ricci, F., Soh, H., et al. (2012). Quantification of Transcription Factor Binding in Cell Extracts Using an Electrochemical, Structure-Switching Biosensor. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(7), 3346-3348 [10.1021/ja2115663].

Quantification of Transcription Factor Binding in Cell Extracts Using an Electrochemical, Structure-Switching Biosensor

RICCI, FRANCESCO;
2012

Abstract

Transcription factor expression levels, which sensitively reflect cellular development and disease state, are typically monitored via cumbersome, reagent-intensive assays that require relatively large quantities of cells. Here, we demonstrate a simple, quantitative approach to their detection based on a simple, electrochemical sensing platform. This sensor sensitively and quantitatively detects its target transcription factor in complex media (e.g., 250 μg/mL crude nuclear extracts) in a convenient, low-reagent process requiring only 10 μL of sample. Our approach thus appears a promising means of monitoring transcription factor levels.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - Chimica Analitica
English
Con Impact Factor ISI
Bonham, A., Hsieh, K., Ferguson, B., Vallée Bélisle, A., Ricci, F., Soh, H., et al. (2012). Quantification of Transcription Factor Binding in Cell Extracts Using an Electrochemical, Structure-Switching Biosensor. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(7), 3346-3348 [10.1021/ja2115663].
Bonham, A; Hsieh, K; Ferguson, B; Vallée Bélisle, A; Ricci, F; Soh, H; Plaxco, K
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/84616
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