The population-shift mechanism can be used for rational re-engineering of structure-switching biosensors to enable their allosteric inhibition and activation. As a proof-of-principle example of this, we have introduced distal allosteric sites into molecular beacons, which are optical sensors for the detection of specific nucleic acid sequences. The binding of inhibitors and activators to these sites enabled the rational modulation of the sensor’s target affinity—and thus its useful dynamic range—over 3 orders of magnitude. The convenience with which this was done suggests that the population-shift mechanism may prove to be a useful method by which allosteric regulation can be introduced into biosensors, “smart” biomaterials, and other artificial biotechnologies.

Ricci, F., Vallée Bélisle, A., Porchetta, A., Plaxco, K. (2012). Rational Design of Allosteric Inhibitors and Activators Using the Population-Shift Model: In Vitro Validation and Application to an Artificial Biosensor. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(37), 15177-15180 [10.1021/ja304672h].

Rational Design of Allosteric Inhibitors and Activators Using the Population-Shift Model: In Vitro Validation and Application to an Artificial Biosensor

RICCI, FRANCESCO;Porchetta, A;
2012-01-01

Abstract

The population-shift mechanism can be used for rational re-engineering of structure-switching biosensors to enable their allosteric inhibition and activation. As a proof-of-principle example of this, we have introduced distal allosteric sites into molecular beacons, which are optical sensors for the detection of specific nucleic acid sequences. The binding of inhibitors and activators to these sites enabled the rational modulation of the sensor’s target affinity—and thus its useful dynamic range—over 3 orders of magnitude. The convenience with which this was done suggests that the population-shift mechanism may prove to be a useful method by which allosteric regulation can be introduced into biosensors, “smart” biomaterials, and other artificial biotechnologies.
2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
Ricci, F., Vallée Bélisle, A., Porchetta, A., Plaxco, K. (2012). Rational Design of Allosteric Inhibitors and Activators Using the Population-Shift Model: In Vitro Validation and Application to an Artificial Biosensor. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(37), 15177-15180 [10.1021/ja304672h].
Ricci, F; Vallée Bélisle, A; Porchetta, A; Plaxco, K
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/84612
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