Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases

Bongiorno Borbone, L., Kadare, G., Benfenati, F., & Girault, J.A. (2005). FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 337(2), 641-646 [10.1016/j.bbrc.2005.09.099].

FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3

BONGIORNO BORBONE, LUCILLA;
2005

Abstract

Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
eng
Con Impact Factor ISI
GKAP; neurons; non-receptor tyrosine kinase; post-synaptic densities; protein-protein interaction; src; synapses; focal adhesion kinase; glutathione transferase; animal tissue; animal cell; nerve cell plasticity; protein function; protein localization; density gradient
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4H68R5D-4-1&_cdi=6713&_user=2606776&_pii=S0006291X05021066&_origin=browse&_zone=rslt_list_item&_coverDate=11/18/2005&_sk=996629997&wchp=dGLbVzW-zSkzV&md5=b53a136be83dd9c7f2563552a3efdba4&ie=/sdarticle.pdf
Bongiorno Borbone, L., Kadare, G., Benfenati, F., & Girault, J.A. (2005). FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 337(2), 641-646 [10.1016/j.bbrc.2005.09.099].
BONGIORNO BORBONE, L; Kadare, G; Benfenati, F; Girault, J
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/8029
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