A mono-enzymatic acetylcholinesterase (AChE) amperometric biosensor for organophosphate detection was developed immobilizing the AChE enzyme via glutaraldehyde on a preformed cysteamine selfassembled monolayer (SAM) on gold-screen printed electrodes (Au-SPEs). The enzymatic activity was monitored measuring the enzymatic product, thiocholine, at an applied potential of +400 mV vs. Ag/AgCl using ferricyanide in solution as electrochemical mediator. The electrocatalytic activity of ferricyanide towards thiocholine was investigated by using Nicholson–Shain method and finding a second order homogenous rate constant ksequal to (5.26 ± 0.65) × 10 4M−1 s −1 . In order to develop a sensitive biosensor, the effect of cysteamine concentration, duration of SAM deposition and AChE concentration were optimized. Using paraoxon as model compound, the biosensor showed a linear range up to 40 ppb with a detection limit of 2 ppb (10% of inhibition). The biosensor was successfully challenged with drinking water sample demonstrating to be a useful analytical tool for organophosphorus insecticide detection.

Arduini, F., Guidone, S., Amine, A., Palleschi, G., MOSCONE DINIA, D. (2013). Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection. SENSORS AND ACTUATORS. B, CHEMICAL, 179, 201-208 [10.1016/j.snb.2012.10.016].

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

ARDUINI, FABIANA;PALLESCHI, GIUSEPPE;MOSCONE DINIA, DANILA
2013-01-01

Abstract

A mono-enzymatic acetylcholinesterase (AChE) amperometric biosensor for organophosphate detection was developed immobilizing the AChE enzyme via glutaraldehyde on a preformed cysteamine selfassembled monolayer (SAM) on gold-screen printed electrodes (Au-SPEs). The enzymatic activity was monitored measuring the enzymatic product, thiocholine, at an applied potential of +400 mV vs. Ag/AgCl using ferricyanide in solution as electrochemical mediator. The electrocatalytic activity of ferricyanide towards thiocholine was investigated by using Nicholson–Shain method and finding a second order homogenous rate constant ksequal to (5.26 ± 0.65) × 10 4M−1 s −1 . In order to develop a sensitive biosensor, the effect of cysteamine concentration, duration of SAM deposition and AChE concentration were optimized. Using paraoxon as model compound, the biosensor showed a linear range up to 40 ppb with a detection limit of 2 ppb (10% of inhibition). The biosensor was successfully challenged with drinking water sample demonstrating to be a useful analytical tool for organophosphorus insecticide detection.
2013
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
acetylcholinesterase; pesticide; organophosphate; self-assembled monolayer; gold screen-printed electrode
Arduini, F., Guidone, S., Amine, A., Palleschi, G., MOSCONE DINIA, D. (2013). Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection. SENSORS AND ACTUATORS. B, CHEMICAL, 179, 201-208 [10.1016/j.snb.2012.10.016].
Arduini, F; Guidone, S; Amine, A; Palleschi, G; MOSCONE DINIA, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/79467
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